@article{oai:iwate-u.repo.nii.ac.jp:00009332,
 author = {矢島, 愛子 and 小向, 奈津美 and 小林, 沙織 and 神志那, 弘明 and 大石, 明広 and 佐藤, れえ子 and 安田, 準},
 issue = {3},
 journal = {動物臨床医学},
 month = {Sep},
 note = {We investigated whether canine serum creatine kinase isoenzyme BB(CK-BB)could be measured by the reagents prepared for human creatine kinase isoenzyme MB (CK-MB), according to an immuno-inhibition method. First,canine serum from a healthy dog and a dog with cerebro-neural-disease was treated respectively with mouse anti-human creatine kinase isoenzyme MM(CK-MM) monoclonal antibody(M antibody) preparation, or with normal mouse immunogloglobulin G(IgG) antibody preparation as a negative control. These treated canine serums and an untreated canine serum were analyzed by agarose electrophoresis/CK enzyme staining.In both
dogs, CK-BB and CK-MM bands were detected in the negative controls and in the untreated serum; only the CK-BB band was seen in the M antibody-treated serums.Further, step-wise diluted samples from serum of a healthy
dog and a CK-MB control solution were measured by the immuno-inhibition method, which resulted in good linearity of calibration curves. In addition, using the serums of 28 tested dogs including dogs with cerebro-neural disease, the results obtained by the immuno-inhibition method and electrophoresis were compared. There was a statistically significant correlation between the residual CK(which was left uninhibited by M antibodies) determined by the immuno-inhibition method and CK-BB activity determined by electrophoresis. There was also a significant correlation between the values of CK-MM activity determined by both methods. We concluded that measurement of canine serum CK-BB by the immuno-inhibition method developed for human clinics is useful in diagnostic
screening for canine cerebro-neural diseases., ヒト用免疫阻害法CK一MB測定用試薬を用いて,犬血清CK-BBを測定できるかを検討した｡犬血清をマウス抗ヒトCK-MMモノクローナル抗体(M抗体)と正常マウスIgG抗体(陰性コントロール)にそれぞれ感作させた後,無処理犬血清と共にアガロース電気泳動･CK酵素染色した｡無処理血清および陰性コントロール血清では,CK-BBとCK-MMが認められた｡M抗体処理血清ではCK-BBは出現したがCK-MMは消失して阻害効果を認めた｡次に,犬血清とCK-MB活性値が既知のコントロール液の段階希釈検体を免疫阻害法で測定したところ,双方の検量線は良好な直線性が認めら
れた｡さらに28頭の犬を用いて,免疫阻害法と電気泳動法において,M抗体で阻害されない残存CKとCK-BBの間および両方の測定方法で得たCK-MMの間で良好な相関関係を認めた｡犬でも免疫阻害法にてCK-BBを評価できることから,犬の神経疾患のスクリーニング検査に用いることができると考えられた｡},
 pages = {79--86},
 title = {免疫阻害法を用いた犬血清クレアチンキナーゼBBアイソザイム(CK-BB)測定法の検討},
 volume = {19},
 year = {2010}
}