{"created":"2023-05-15T12:04:45.435127+00:00","id":9332,"links":{},"metadata":{"_buckets":{"deposit":"660ed465-92d2-490c-8e28-c3788a05cb83"},"_deposit":{"created_by":3,"id":"9332","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"9332"},"status":"published"},"_oai":{"id":"oai:iwate-u.repo.nii.ac.jp:00009332","sets":["1504:1510"]},"author_link":["72884","72888","72891","72894","72887","72890","72895","72893","72896","72892","72889","72883","72885","72886"],"item_16_alternative_title_23":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Evaluation of Measuring Canine Serum Creatine Kinase Isoenzyme BB by an Immuno-inhibition Method"}]},"item_16_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-09-20","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"86","bibliographicPageStart":"79","bibliographicVolumeNumber":"19","bibliographic_titles":[{"bibliographic_title":"動物臨床医学"}]}]},"item_16_date_6":{"attribute_name":"登録日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2012-02-14"}]},"item_16_description_12":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"We investigated whether canine serum creatine kinase isoenzyme BB(CK-BB)could be measured by the reagents prepared for human creatine kinase isoenzyme MB (CK-MB), according to an immuno-inhibition method. First,canine serum from a healthy dog and a dog with cerebro-neural-disease was treated respectively with mouse anti-human creatine kinase isoenzyme MM(CK-MM) monoclonal antibody(M antibody) preparation, or with normal mouse immunogloglobulin G(IgG) antibody preparation as a negative control. These treated canine serums and an untreated canine serum were analyzed by agarose electrophoresis/CK enzyme staining.In both\ndogs, CK-BB and CK-MM bands were detected in the negative controls and in the untreated serum; only the CK-BB band was seen in the M antibody-treated serums.Further, step-wise diluted samples from serum of a healthy\ndog and a CK-MB control solution were measured by the immuno-inhibition method, which resulted in good linearity of calibration curves. In addition, using the serums of 28 tested dogs including dogs with cerebro-neural disease, the results obtained by the immuno-inhibition method and electrophoresis were compared. There was a statistically significant correlation between the residual CK(which was left uninhibited by M antibodies) determined by the immuno-inhibition method and CK-BB activity determined by electrophoresis. There was also a significant correlation between the values of CK-MM activity determined by both methods. We concluded that measurement of canine serum CK-BB by the immuno-inhibition method developed for human clinics is useful in diagnostic\nscreening for canine cerebro-neural diseases.","subitem_description_type":"Other"},{"subitem_description":"ヒト用免疫阻害法CK一MB測定用試薬を用いて,犬血清CK-BBを測定できるかを検討した｡犬血清をマウス抗ヒトCK-MMモノクローナル抗体(M抗体)と正常マウスIgG抗体(陰性コントロール)にそれぞれ感作させた後,無処理犬血清と共にアガロース電気泳動･CK酵素染色した｡無処理血清および陰性コントロール血清では,CK-BBとCK-MMが認められた｡M抗体処理血清ではCK-BBは出現したがCK-MMは消失して阻害効果を認めた｡次に,犬血清とCK-MB活性値が既知のコントロール液の段階希釈検体を免疫阻害法で測定したところ,双方の検量線は良好な直線性が認めら\nれた｡さらに28頭の犬を用いて,免疫阻害法と電気泳動法において,M抗体で阻害されない残存CKとCK-BBの間および両方の測定方法で得たCK-MMの間で良好な相関関係を認めた｡犬でも免疫阻害法にてCK-BBを評価できることから,犬の神経疾患のスクリーニング検査に用いることができると考えられた｡","subitem_description_type":"Other"}]},"item_16_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"72890","nameIdentifierScheme":"WEKO"}],"names":[{"name":"YAJIMA, Aiko"}]},{"nameIdentifiers":[{"nameIdentifier":"72891","nameIdentifierScheme":"WEKO"}],"names":[{"name":"KOMUKAI, Natsumi"}]},{"nameIdentifiers":[{"nameIdentifier":"72892","nameIdentifierScheme":"WEKO"}],"names":[{"name":"KOBAYASHI, Saori"}]},{"nameIdentifiers":[{"nameIdentifier":"72893","nameIdentifierScheme":"WEKO"}],"names":[{"name":"KAMISHINA, Hiroaki"}]},{"nameIdentifiers":[{"nameIdentifier":"72894","nameIdentifierScheme":"WEKO"}],"names":[{"name":"OISHI, Akihiro"}]},{"nameIdentifiers":[{"nameIdentifier":"72895","nameIdentifierScheme":"WEKO"}],"names":[{"name":"SATO, Reeko"}]},{"nameIdentifiers":[{"nameIdentifier":"72896","nameIdentifierScheme":"WEKO"}],"names":[{"name":"YASUDA, Jun"}]}]},"item_16_publisher_14":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"動物臨床医学会"}]},"item_16_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1344-6991","subitem_source_identifier_type":"ISSN"}]},"item_16_text_4":{"attribute_name":"著者(機関)","attribute_value_mlt":[{"subitem_text_value":"岩手大学農学部獣医学課程小動物内科学研究室"}]},"item_16_version_type_27":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"矢島, 愛子"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"小向, 奈津美"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"小林, 沙織"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"神志那, 弘明"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"大石, 明広"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"佐藤, れえ子"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"安田, 準"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-11-14"}],"displaytype":"detail","filename":"jacm-v19n3p79-86.pdf","filesize":[{"value":"467.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"jacm-v19n3p79-86.pdf","url":"https://iwate-u.repo.nii.ac.jp/record/9332/files/jacm-v19n3p79-86.pdf"},"version_id":"89c7836b-ffc8-42da-9136-ee229ec6468c"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"BB isoenzyme","subitem_subject_scheme":"Other"},{"subitem_subject":"canine serum","subitem_subject_scheme":"Other"},{"subitem_subject":"cerebro-neural diseases","subitem_subject_scheme":"Other"},{"subitem_subject":"creatine kinase","subitem_subject_scheme":"Other"},{"subitem_subject":"immuno-inhibition method","subitem_subject_scheme":"Other"},{"subitem_subject":"BBアイソザイム","subitem_subject_scheme":"Other"},{"subitem_subject":"犬血清","subitem_subject_scheme":"Other"},{"subitem_subject":"脳神経疾患","subitem_subject_scheme":"Other"},{"subitem_subject":"クレアチンキナーゼ","subitem_subject_scheme":"Other"},{"subitem_subject":"免疫阻害法","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"免疫阻害法を用いた犬血清クレアチンキナーゼBBアイソザイム(CK-BB)測定法の検討","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"免疫阻害法を用いた犬血清クレアチンキナーゼBBアイソザイム(CK-BB)測定法の検討"}]},"item_type_id":"16","owner":"3","path":["1510"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-02-14"},"publish_date":"2012-02-14","publish_status":"0","recid":"9332","relation_version_is_last":true,"title":["免疫阻害法を用いた犬血清クレアチンキナーゼBBアイソザイム(CK-BB)測定法の検討"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-05-16T11:05:37.440316+00:00"}