{"created":"2023-05-15T12:04:45.904718+00:00","id":9339,"links":{},"metadata":{"_buckets":{"deposit":"36f8c41b-6721-4899-b296-a7e22657b988"},"_deposit":{"created_by":3,"id":"9339","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"9339"},"status":"published"},"_oai":{"id":"oai:iwate-u.repo.nii.ac.jp:00009339","sets":["1504:1510"]},"author_link":["72949","72956","72963","72950","72962","72964","72947","72955","72960","72951","72961","72965","72957","72953","72966","72959","72958","72952","72948","72954"],"item_16_alternative_title_23":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"アサガオの speckled 変異と c-1 変異によるアントシアニンの制御"}]},"item_16_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-01-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"460","bibliographicPageStart":"452","bibliographicVolumeNumber":"80","bibliographic_titles":[{"bibliographic_title":"Journal of the Japanese Society for Horticultural Science"}]}]},"item_16_date_6":{"attribute_name":"登録日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2012-02-24"}]},"item_16_description_12":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"Anthocyanin pigments and their related compounds were examined in the flowers of F1 and F2 plants generated by crosses between two acyanic lines, 54Y having a speckled mutation for pale yellow flowers and 78WWc-1 carrying the c-1 mutation conferring white flowers, in Japanese morning glory. The mutable speckled allele generates speckled flowers when a dominant genetic element, speckled-activator, is present in its genome. The Speckled and C-1 loci are tightly linked, and 78WWc-1, but not 54Y, bears the specked-activator. The flower color of F1 progeny is red-purple, and F2 population displays a ratio of 8 red-purple flowers: 4 white flowers: 3 speckled flowers with red-purple spots pale-yellow background: 1 pale-yellow flower. The anthocyanin components of red-purple flowers in F1 and F2 plants as well as red-purple spots of the speckled flower in the F2 generation were identical. Wedding Bells Anthocyanin (WBA) was accumulated in red-purple flowers as the dominant anthocyanin along with its precursor, pelargonidin 3-sophoroside-5-glucoside, and eight of its acylated derivatives as minor anthocyanins. The major flavonoid pigment in the pale-yellow flowers of 54Y and the F2 plants as well as the pale-yellow background of the speckled flower in the F2 generation was identified as chalcononaringenin 2'-glucoside by chemical and spectroscopic methods. Furthermore, chlorogenic acid and small amounts of caffeic acid as well as aureusidin 4-glucoside were obtained from the pale-yellow flowers. Chlorogenic acid and caffeic acid were also detected in the white flowers of F2 and 78WWc-1. These results suggest that anthocyanin biosynthesis in speckled and c1 mutants stopped at the steps mediated by chalcone isomerase (CHI) and chalcone synthase (CHS), respectively, and that anthocyanin biosynthesis was completely recovered in the red-purple tissue parts of the speckled flower. Moreover, we found that anthocyanin composition varied, especially in the red-purple flowers and red-purple tissue parts in the speckled flowers of F2 plants, implying that WBA biosynthesis depends on unknown genetic backgrounds.","subitem_description_type":"Other"},{"subitem_description":"アサガオ（Ipomoea nil または，Pharbitis nil）の speckled 変異により淡黄色花を咲かせる 54Y 系統と c-1 変異により白花を持つ 78WWc-1 系統の F1 並びに F2 植物について，花弁に含まれるアントシアニンとその関連化合物を解析した．speckled 変異が優性の遺伝因子である speckled-activator と共存すると，花弁に吹掛絞が現れる．Speckled と C-1 遺伝子座は強く連鎖しており，78WWc-1 系統にのみ speckled-activator が存在する．このため，F1 植物は赤紫花を咲かせ，F2 では赤紫花，白花，吹掛絞（淡黄色地に赤紫の斑点模様）の花，淡黄色花の植物が 8 : 4 : 3 : 1 の割合で分離する．解析の結果，F1 と F2 の赤紫花，さらに吹掛絞の斑点部分には同じアントシアニンが含まれていた．いずれもウェディングベルアントシアニン（WBA）が主要な色素であり，その前駆体など 9 種のアントシアニン（ペラルゴニジン誘導体）も蓄積していた．一方，54Y と淡黄色花の F2，さらに吹掛絞の淡黄の地色部分では，カルコノナリンゲニン 2'-グルコシドが主要なフラボノイドとして検出されたほか，少量のカフェ酸とオーロシジン 4-グルコシドやクロロゲン酸もみいだされた．また，78WWc-1 と白花の F2 には，クロロゲン酸とカフェ酸が存在した．これらの結果から，speckled 変異と c-1 変異を持つアサガオでは，それぞれカルコン異性化酵素（CHI）とカルコン合成酵素（CHS）が触媒する反応過程でアントシアニン色素生合成が遮断されていることが強く示唆された．また，吹掛絞の斑点部分では，完全に色素生合成系が活性化していると思われた．さらに，F2 の赤紫花と吹掛絞の斑点部分に含まれるアントシアニン構成が個体毎に異なることから，これまで報告されていない未知の遺伝的背景が WBA の生合成を制御している可能性が高い．","subitem_description_type":"Other"}]},"item_16_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"72957","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Saito, Norio"}]},{"nameIdentifiers":[{"nameIdentifier":"72958","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Tatsuzawa, Fumi"}]},{"nameIdentifiers":[{"nameIdentifier":"72959","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Hoshino, Atsushi"}]},{"nameIdentifiers":[{"nameIdentifier":"72960","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Abe, Yukihide"}]},{"nameIdentifiers":[{"nameIdentifier":"72961","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Ichimura, Michiyo"}]},{"nameIdentifiers":[{"nameIdentifier":"72962","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Yokoi, Masato"}]},{"nameIdentifiers":[{"nameIdentifier":"72963","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Toki, Kenjiro"}]},{"nameIdentifiers":[{"nameIdentifier":"72964","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Morita, Yasumasa"}]},{"nameIdentifiers":[{"nameIdentifier":"72965","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Iida, Shigeru"}]},{"nameIdentifiers":[{"nameIdentifier":"72966","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Honda, Toshio"}]}]},"item_16_publisher_14":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Japanese Society for Horticultural Science"}]},"item_16_relation_26":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.2503/jjshs1.80.452","subitem_relation_type_select":"DOI"}}]},"item_16_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1882-3351","subitem_source_identifier_type":"ISSN"}]},"item_16_text_4":{"attribute_name":"著者(機関)","attribute_value_mlt":[{"subitem_text_value":"Faculty of Pharmaceutical Sciences, Hoshi University"},{"subitem_text_value":"Faculty of Agriculture, Iwate University"},{"subitem_text_value":"National Institute for Basic Biology"},{"subitem_text_value":"College of Economics, Nihon University"},{"subitem_text_value":"Faculty of Horticulture, Chiba University"},{"subitem_text_value":"Faculty of Horticulture, Chiba University"},{"subitem_text_value":"Faculty of Horticulture, Minami-Kyushu University"},{"subitem_text_value":"National Institute for Basic Biology"},{"subitem_text_value":"National Institute for Basic Biology"},{"subitem_text_value":"Faculty of Pharmaceutical Sciences, Hoshi University"}]},"item_16_version_type_27":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"斎藤, 規夫"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"立澤, 文見"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"星野, 敦"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"阿部, 幸穎"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"市村, 美千代"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"横井, 政人"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"土岐, 健次郎"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"森田, 裕将"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"飯田, 滋"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"本多, 利雄"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-11-14"}],"displaytype":"detail","filename":"jjshs-v80n4p452-460.pdf","filesize":[{"value":"359.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"jjshs-v80n4p452-460.pdf","url":"https://iwate-u.repo.nii.ac.jp/record/9339/files/jjshs-v80n4p452-460.pdf"},"version_id":"88ad73f3-c897-44cc-a7d1-9924b2539a5c"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"acylated pelargonidin glycoside","subitem_subject_scheme":"Other"},{"subitem_subject":"anthocyanin biosynthesis","subitem_subject_scheme":"Other"},{"subitem_subject":"chalcone isomerase","subitem_subject_scheme":"Other"},{"subitem_subject":"Japanese morning glory","subitem_subject_scheme":"Other"},{"subitem_subject":"speckled flower pigmentation","subitem_subject_scheme":"Other"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Anthocyanin Pigmentation Controlled by speckled and c-1 Mutations of Japanese Morning Glory","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Anthocyanin Pigmentation Controlled by speckled and c-1 Mutations of Japanese Morning Glory"}]},"item_type_id":"16","owner":"3","path":["1510"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-02-24"},"publish_date":"2012-02-24","publish_status":"0","recid":"9339","relation_version_is_last":true,"title":["Anthocyanin Pigmentation Controlled by speckled and c-1 Mutations of Japanese Morning Glory"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-05-16T11:05:17.267374+00:00"}