@article{oai:iwate-u.repo.nii.ac.jp:00009386,
 author = {Watanabe, Hitoshi and Saito, Ryo and Nakano, Tatsuya and Takahashi, Hideyuki and Takahashi, Yu and Sumiyoshi, Keisuke and Sato, Katsuyoshi and Chen, Xiangning and Okada, Natsumi and Iwasaki, Shunsuke and Harjanti, Dian W. and Sekiguchi, Natsumi and Sano, Hiroaki and Kitazawa, Haruki and Rose, Michael T. and Ohwada, Shyuichi and Watanabe, Kouichi and Aso, Hisashi},
 issue = {2},
 journal = {PLOS ONE},
 month = {Feb},
 note = {In mice, peripheral 5-HT induces an increase in the plasma concentrations of glucose, insulin and bile acids, and a decrease
in plasma triglyceride, NEFA and cholesterol concentrations. However, given the unique characteristics of the metabolism of
ruminants relative to monogastric animals, the physiological role of peripheral 5-HT on glucose and lipid metabolism in
sheep remains to be established. Therefore, in this study, we investigated the effect of 5-HT on the circulating
concentrations of metabolites and insulin using five 5-HT receptor (5HTR) antagonists in sheep. After fasting for 24 h, sheep
were intravenously injected with 5-HT, following which-, plasma glucose, insulin, triglyceride and NEFA concentrations were
significantly elevated. In contrast, 5-HT did not affect the plasma cholesterol concentration, and it induced a decrease in bile
acid concentrations. Increases in plasma glucose and insulin concentrations induced by 5-HT were attenuated by pretreatment
with Methysergide, a 5HTR 1, 2 and 7 antagonist. Additionally, decreased plasma bile acid concentrations induced
by 5-HT were blocked by pre-treatment with Ketanserin, a 5HTR 2A antagonist. However, none of the 5HTR antagonists
inhibited the increase in plasma triglyceride and NEFA levels induced by 5-HT. On the other hand, mRNA expressions of
5HTR1D and 1E were observed in the liver, pancreas and skeletal muscle. These results suggest that there are a number of
differences in the physiological functions of peripheral 5-HT with respect to lipid metabolism between mice and sheep,
though its effect on glucose metabolism appears to be similar between these species.},
 title = {Effect of Peripheral 5-HT on Glucose and Lipid Metabolism in Wether Sheep},
 volume = {9},
 year = {2014}
}