@article{oai:iwate-u.repo.nii.ac.jp:00009387,
 author = {福岡, 寛之 and 佐々木, 淳 and 岡田, 幸助 and 川崎, 武志 and 御領, 政信},
 issue = {3},
 journal = {鶏病研究会報},
 month = {Jan},
 note = {死亡率の上昇とともに剖検時に肝臓の腫大・退色および大きな卵黄嚢の遺残が認められた16日齢ブロイラーひなにおいて，病理組織学的に封入体肝炎の他に卵黄嚢上皮細胞に好塩基性核内封入体が形成されていた。死亡鶏の凍結肝臓より作製した肝乳剤を鶏腎細胞に接種したところ，好塩基性核内封入体を形成するウイルス(8754株，8755株)が分離され，3代継代したのち，Line-M系由来のSPF初生ひなを用いて再現実験を行った。接種後6日目で全ひなが死亡し，肉眼的に肝臓では腫大・脆弱化および点状出血が認められた。病理組織学的に肝臓，卵黄嚢，膵臓，十二指腸粘膜上皮細胞において核内封入体の形成が認められた。肝臓の電子顕微鏡観察では，肝細胞の核内に直径約70～80nmの結晶状配列をするウイルス粒子がみられ，8754株に対する鶏血清を用いた間接蛍光抗体法では，肝臓および卵黄嚢に形成された核内封入体に一致して黄緑色の蛍光が観察された。以上の所見より，　卵黄嚢上皮細胞がトリアデノウイルスの標的細胞となりえることが初めて証明された。, In addition to inclusion body hepatitis, basophilic intranuclear inclusion bodies were histologically observed in the yolk sac epithelium of 16-day broiler chicks with increased mortality of chicken flocks. Two viruses (tentatively designated as strains 8754 and 8755) were isolated in a chicken kidney cell culture from the liver of affected chickens and passaged three times. One-day-old chicks derived from SPF Line-M were inoculated with strain 8754 or 8755 for reproduction of the disease. All chicks were dead within six days post inoculation, and their livers were swollen and fragile with petechial hemorrhages. 
Histologically, intranuclear inclusion bodies were observed in the hepatocytes, yolk sac epithelium, pancreatic acinar cells, and enterocytes of the small intestine. Ultrastructurally, 70 to 80 nm diameter virus particles with crystalline arrays were observed in the swollen nucleus of hepatocytes. In an indirect fluorescent antibody test, yellow-green fluorescence corresponded with intranuclear inclusion bodies in hepatocytes and yolk sac epithelium. These results suggest that the yolk sac epithelium may be a target of avian adenovirus.},
 pages = {181--187},
 title = {ブロイラーひなの卵黄嚢上皮細胞に感染性を示すトリアデノウイルス感染症},
 volume = {46},
 year = {2010}
}