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Lysine suppresses myofibrillar protein degradation by regulating the autophagic-lysosomal system through phosphorylation of Akt in C2C12 cells
https://iwate-u.repo.nii.ac.jp/records/9417
https://iwate-u.repo.nii.ac.jp/records/9417eabb06cd-c996-4f1d-96f8-6b5f2173c5ea
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
sp-3;584p1-12.pdf (1.3 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||
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| 公開日 | 2014-12-17 | |||||||||||
| タイトル | ||||||||||||
| タイトル | Lysine suppresses myofibrillar protein degradation by regulating the autophagic-lysosomal system through phosphorylation of Akt in C2C12 cells | |||||||||||
| 言語 | ||||||||||||
| 言語 | eng | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Lysine | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Proteolysis | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | C2C12 | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Akt | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | AMPK | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
| 資源タイプ | journal article | |||||||||||
| 著者 |
Sato, Tomonori
× Sato, Tomonori
× Ito, Yoshiaki
× Nagasawa, Takashi
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| 著者(機関) | ||||||||||||
| 値 | Department of Bioresources Science, The United Graduate School of Agricultural Sciences, Iwate University | |||||||||||
| 著者(機関) | ||||||||||||
| 値 | Department of Biological Chemistry and Food Science, Graduate School of Agriculture, Iwate University | |||||||||||
| 著者(機関) | ||||||||||||
| 値 | Department of Biological Chemistry and Food Science, Graduate School of Agriculture, Iwate University | |||||||||||
| 登録日 | ||||||||||||
| 日付 | 2014-12-17 | |||||||||||
| 書誌情報 |
SpringerPlus 巻 3, 号 584, p. 1-12, 発行日 2014-10-08 |
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| Abstract | ||||||||||||
| 内容記述タイプ | Other | |||||||||||
| 内容記述 | The prevention of muscle wasting is important for maintaining quality of life, since loss of muscle mass can lead to a bedridden state and decreased resistance to diseases. The prevention of muscle wasting requires an increase in protein synthesis and a decrease in protein degradation in skeletal muscle. We previously showed that lysine (Lys) markedly suppressed myofibrillar protein degradation by inhibiting the autophagic-lysosomal system via the mammalian target of rapamycin (mTOR) and other signal molecules in C2C12 cells. In this study, we investigated the involvement of Akt and adenosine 5′-monophosphate (AMP)-activated protein kinase (AMPK), two regulators of autophagy, on the suppressive effects of Lys on myofibrillar protein degradation in C2C12 cells. Lys induced the phosphorylation of Akt, but the suppressive effects of Lys on myofibrillar protein degradation and autophagy were completely abolished in the presence of Akt1/2 kinase inhibitor (Akti). Lys suppressed the phosphorylation of AMPK, but this effect was also abolished by Akti. On the other hand, AMPK activation by -aminoimidazole-4-carboxamide- 1-β-D-ribonucleoside (AICAR) did not affect either Akt activity or the autophagic-lysosomal system in C2C12 cells treated with Lys. These results indicate that regulation of AMPK activity is not essential for the regulation of autophagy by Lys. Taken together, our results show that Lys suppresses myofibrillar protein degradation by the autophagic-lysosomal system through the phosphorylation of Akt in C2C12 cells. |
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| 出版者 | ||||||||||||
| 出版者 | Springer | |||||||||||
| 権利 | ||||||||||||
| 権利情報 | © 2014 Sato et al.; licensee Springer. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. | |||||||||||
| DOI | ||||||||||||
| 関連タイプ | isIdenticalTo | |||||||||||
| 識別子タイプ | DOI | |||||||||||
| 関連識別子 | 10.1186/2193-1801-3-584 | |||||||||||
| 著者版フラグ | ||||||||||||
| 出版タイプ | VoR | |||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||
