| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2015-02-24 |
| タイトル |
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|
タイトル |
Monitoring and kinetic analysis of the molecular interactions by which a repressor protein, PhaR, binds to target DNAs and poly[(R)-3-hydroxybutyrate] |
| 言語 |
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|
言語 |
eng |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Polyhydroxyalkanoate |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Autoregulator protein PhaR |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Kinetic analysis |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Ralstonia eutropha H16 |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Yamada, Miwa
Takahashi, Shuntaro
Okahata, Yoshio
Doi, Yoshiharu
Numata, Keiji
|
| 著者(機関) |
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|
値 |
Enzyme Research Team, RIKEN Biomass Engineering Program, RIKEN, Department of Biological Chemistry and Food Science, Faculty of Agriculture, Iwate University |
| 著者(機関) |
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|
値 |
Department of Biomolecular Engineering, Tokyo Institute of Technology, Frontier Institute for Biomolecular Engineering Research (FIBER), Konan University |
| 著者(機関) |
|
|
値 |
Department of Biomolecular Engineering, Tokyo Institute of Technology |
| 著者(機関) |
|
|
値 |
Research Cluster for Innovation, RIKEN |
| 著者(機関) |
|
|
値 |
Enzyme Research Team, RIKEN Biomass Engineering Program, RIKEN |
| 登録日 |
|
|
日付 |
2015-02-24 |
| 書誌情報 |
AMB Express
巻 3,
号 6,
発行日 2013-01-01
|
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
2191-0855 |
| Abstract |
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内容記述タイプ |
Other |
|
内容記述 |
The repressor protein PhaR, which is a component of poly[(R)-3-hydroxybutyrate] granules, functions as a repressor of the gene expression of the phasin PhaP and of PhaR itself. We used a quartz crystal microbalance to investigate the binding behavior by which PhaR in Ralstonia eutropha H16 targets DNAs and amorphous poly[(R)-3-hydroxybutyrate] thin films. Binding rate constants, dissociation rate constants, and dissociation constants of the binding of PhaR to DNA and to amorphous poly[(R)-3-hydroxybutyrate] suggested that PhaR bind to both in a similar manner. On the basis of the binding rate constant values, we proposed that the phaP gene would be derepressed in harmony with the ratio of the concentration of the target DNA to the concentration of amorphous poly[(R)-3-hydroxybutyrate] at the start of poly[(R)-3-hydroxybutyrate] synthesis in R. eutropha H16. |
| 出版者 |
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出版者 |
Springer-Verlag |
| 権利 |
|
|
権利情報 |
© 2013 Yamada et al.; licensee Springer |
| 権利URI |
|
|
権利情報 |
http://creativecommons.org/licenses/by/2.0/ |
| DOI |
|
|
関連タイプ |
isIdenticalTo |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1186/2191-0855-3-6 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
ambe-v3n6.pdf (544.6 kB)
