{"created":"2023-05-15T12:05:13.531700+00:00","id":9849,"links":{},"metadata":{"_buckets":{"deposit":"2dd0e1be-bdbf-4c88-95f0-646b8c908a85"},"_deposit":{"created_by":3,"id":"9849","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"9849"},"status":"published"},"_oai":{"id":"oai:iwate-u.repo.nii.ac.jp:00009849","sets":["1515:1519"]},"author_link":["61172","61175","61171","61179","61170","61174","61173","61177","61176","61178"],"item_16_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-01-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"19","bibliographicPageStart":"15","bibliographicVolumeNumber":"58","bibliographic_titles":[{"bibliographic_title":"分析化学"}]}]},"item_16_date_6":{"attribute_name":"登録日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2009-11-05"}]},"item_16_description_12":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"著者らは，ヒト皮膚の角質中に含まれるカテプシンL（Cat L）をバイオマーカーとした皮膚評価手法の研究を進めている．本論文では，微量な角質Cat Lの簡便な活性分析法の確立を目的とし，角質採取テープを用いた角質の採取，角質サンプルの濃縮，阻害剤と蛍光基質による感度の向上を図った．本分析によるCat L活性は0.003～0.04 U/Lの範囲内においてR2＝0.98という良好な結果が得られた．本分析方法を用いてヒトでの生体評価を行ったところ，角質Cat L活性は0.005～0.028 U/Lの範囲に分布し，本測定法が十分な測定範囲を有していることが示された．このとき，角質Cat L活性と総タンパク量の相関係数は0.09と低い値を示し，角質Cat L活性を皮膚状態の指標として用いるには，単位総タンパク量当たりの角質Cat L活性を求める必要があると考えられた．すなわち，本分析方法を用いることで，角質Cat L活性を高感度，簡便に分析可能であることが示された．","subitem_description_type":"Other"}]},"item_16_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"61175","nameIdentifierScheme":"WEKO"}],"names":[{"name":"TAHARA, Yusuke"}]},{"nameIdentifiers":[{"nameIdentifier":"61176","nameIdentifierScheme":"WEKO"}],"names":[{"name":"DATE, Akira"}]},{"nameIdentifiers":[{"nameIdentifier":"61177","nameIdentifierScheme":"WEKO"}],"names":[{"name":"MAKINO, Teruhiko"}]},{"nameIdentifiers":[{"nameIdentifier":"61178","nameIdentifierScheme":"WEKO"}],"names":[{"name":"SHIMIZU, Tadamichi"}]},{"nameIdentifiers":[{"nameIdentifier":"61179","nameIdentifierScheme":"WEKO"}],"names":[{"name":"YAMAGUCHI, Masaki"}]}]},"item_16_publisher_14":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本分析化学会"}]},"item_16_relation_26":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.2116/bunsekikagaku.58.15","subitem_relation_type_select":"DOI"}}]},"item_16_rights_18":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"The Japan Society for Analytical Chemistry (c) 2009"}]},"item_16_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0525-1931","subitem_source_identifier_type":"ISSN"}]},"item_16_text_4":{"attribute_name":"著者(機関)","attribute_value_mlt":[{"subitem_text_value":"岩手大学大学院工学研究科生産開発工学専攻"},{"subitem_text_value":"プロクター・アンド・ギャンブル・ジャパン株式会社"},{"subitem_text_value":"富山大学大学院医学薬学研究部"},{"subitem_text_value":"富山大学大学院医学薬学研究部"},{"subitem_text_value":"岩手大学大学院工学研究科生産開発工学専攻"}]},"item_16_version_type_27":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"田原, 祐助"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"伊達, 朗"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"牧野, 輝彦"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"清水, 忠道"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"山口, 昌樹"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-11-14"}],"displaytype":"detail","filename":"bk-v58n1p15-19.pdf","filesize":[{"value":"973.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"bk-v58n1p15-19.pdf","url":"https://iwate-u.repo.nii.ac.jp/record/9849/files/bk-v58n1p15-19.pdf"},"version_id":"955c5670-e309-40e3-a01c-5da21ad4ccb8"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"cathepsin L","subitem_subject_scheme":"Other"},{"subitem_subject":"stratum corneum","subitem_subject_scheme":"Other"},{"subitem_subject":"tape stripping","subitem_subject_scheme":"Other"},{"subitem_subject":"fluorescent substrate","subitem_subject_scheme":"Other"},{"subitem_subject":"total protein","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"ヒト皮膚状態評価のための角質のカテプシンL活性分析法","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ヒト皮膚状態評価のための角質のカテプシンL活性分析法"}]},"item_type_id":"16","owner":"3","path":["1519"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-11-05"},"publish_date":"2009-11-05","publish_status":"0","recid":"9849","relation_version_is_last":true,"title":["ヒト皮膚状態評価のための角質のカテプシンL活性分析法"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-05-16T11:55:03.234203+00:00"}