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Detection of Two New Variants of Soybean Kunitz Trypsin Inhibitor through Electrophoresis
https://iwate-u.repo.nii.ac.jp/records/9322
https://iwate-u.repo.nii.ac.jp/records/9322e7d8c341-84fc-4727-a70f-7614c7135f10
名前 / ファイル | ライセンス | アクション |
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bs-v46n1p39-43.pdf (462.4 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2012-01-13 | |||||
タイトル | ||||||
タイトル | Detection of Two New Variants of Soybean Kunitz Trypsin Inhibitor through Electrophoresis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | soybean (Glycine max ) | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | wild soybean (Gly-cine soja ) | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | soybean Kunitz trypsin inhibitor (SKTI) | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | electrophoretic mobility variant | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | frequency distribution of the alleles. | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
土, 克晶
× 土, 克晶× 海妻, 矩彦× 高畑, 義人× 畠山, 茂雄 |
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著者別名 | ||||||
姓名 | WANG, Ke-Jing | |||||
著者別名 | ||||||
姓名 | KAIZUMA, Norihiko | |||||
著者別名 | ||||||
姓名 | TAKAHATA, Yoshihito | |||||
著者別名 | ||||||
姓名 | HATAKEYAMA, Shigeo | |||||
著者(機関) | ||||||
岩手大学農学部 | ||||||
登録日 | ||||||
日付 | 2012-01-13 | |||||
書誌情報 |
Breeding science 巻 46, 号 1, p. 39-44, 発行日 1966-03-01 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1344-7610 | |||||
Abstract | ||||||
内容記述タイプ | Other | |||||
内容記述 | 大豆の種子に含まれるKunitz型トリプシンインヒビター(SKTI)には電気泳動により識別される5種の多型(Tia,TibTic,欠失及び未命名)がある.TiaとTib型の間には8アミノ酸残基,TiaとTic型の間には1アミノ酸残基の違いがあることが知られているが,その中間が知られていない.本研究ではその中間型があるかどうかを知るため,電気泳動の移動度の僅かな違いが識別できるような電気泳動法を工夫し,日本の岩手県の62地点から採集した890ツルマメのサンプル(1991-1992年に採集)および173の日本及び中国の栽培品種を材料として(Tab1e1),Kunitz型トリプシンインヒビタータンパク質の電気泳動移動度の微小変異を示す多型性の有無について調査した. | |||||
Abstract | ||||||
内容記述タイプ | Other | |||||
内容記述 | In order to detect mobility variants of soybean Kunitz trypsin inhibitor (SKTI) proteins, we developed a large slab gel method (18.5 cm long X 14.5 cm wide) of elec-trophoresis, and analyzed 173 cultivars of soybeans (Glycine max ) from Japan and China, and 62 populations of wild soybeans (G. soja ) collected from all over lwate Prefecture, Japan. As a result of this electrophoretic survey, two new mobility variants were discovered: a variant showing a slightly slower mobility than the Tia type (tentatively designated as Tia-s ) in a wild soybean line and another variant with a slightly faster mobility than the Tib type (tentatively designated as Tib-f) in a cultivated soybean variety. Transmissibility of this variation to consecutive generations was confirmed by parent-offspring tests and hybridization tests using the variants and/or the common types. Isoelectric focusing (IEF) test applied to these two variants revealed differences in the location of the isoelet-ric points in the gel. Significance of these discoveries was discussed. | |||||
出版者 | ||||||
出版者 | 日本育種学会 | |||||
権利 | ||||||
権利情報 | 日本育種学会 | |||||
その他のタイトル | ||||||
その他のタイトル | ダイズKunitz型トリプシンインヒビターの2つの新しい多型の電気泳動による検出 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |