| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2021-12-06 |
| タイトル |
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タイトル |
Deficiency of the RIβ subunit of protein kinase A causes body tremor and impaired fear conditioning memory in rats |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Hieu, Hoang Trung
YOSHIHARA, Toru
NAKAO, Akito
HAYASHIDA, Katsumi
HIRATA, Yoshiki
SHIRASUNA, Koumei
KUWAMURA, Mitsuru
NAKAGAWA, Yuki
KANEKO, Takehito
MORI, Yasuo
ASANO, Masahide
KURAMOTO, Takashi
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| 著者(機関) |
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|
値 |
Laboratory of Animal Nutrition, Department of Animal Science, Faculty of Agriculture, Tokyo University of Agriculture |
| 著者(機関) |
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値 |
Institute of Laboratory Animals, Graduate School of Medicine, Kyoto Universit |
| 著者(機関) |
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値 |
Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University |
| 著者(機関) |
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値 |
Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University |
| 著者(機関) |
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値 |
Laboratory of Animal Reproduction, Department of Animal Science, Faculty of Agriculture, Tokyo University of Agriculture |
| 著者(機関) |
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値 |
Laboratory of Animal Reproduction, Department of Animal Science, Faculty of Agriculture, Tokyo University of Agriculture |
| 著者(機関) |
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|
値 |
Laboratory of Veterinary Pathology, Graduate School of Life and Environmental Science, Osaka Prefecture University |
| 著者(機関) |
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値 |
Department of Chemistry and Biological Sciences, Faculty of Science and Engineering, Iwate University |
| 著者(機関) |
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値 |
Department of Chemistry and Biological Sciences, Faculty of Science and Engineering, Iwate University |
| 著者(機関) |
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値 |
Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University |
| 著者(機関) |
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値 |
Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University |
| 著者(機関) |
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|
値 |
Laboratory of Animal Nutrition, Department of Animal Science, Faculty of Agriculture, Tokyo University of Agriculture, Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University |
| 登録日 |
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日付 |
2021-12-07 |
| 書誌情報 |
Scientific Reports
巻 11,
p. 2039,
発行日 2021-01-21
|
| ISSN |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
20452322 |
| 抄録 |
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|
内容記述タイプ |
Abstract |
|
内容記述 |
The RIβ subunit of cAMP-dependent protein kinase (PKA), encoded by Prkar1b, is a neuronal isoform of the type I regulatory subunit of PKA. Mice lacking the RIβ subunit exhibit normal long-term potentiation (LTP) in the Schaffer collateral pathway of the hippocampus and normal behavior in the open-field and fear conditioning tests. Here, we combined genetic, electrophysiological, and behavioral approaches to demonstrate that the RIβ subunit was involved in body tremor, LTP in the Schaffer collateral pathway, and fear conditioning memory in rats. Genetic analysis of WTC-furue, a mutant strain with spontaneous tremors, revealed a deletion in the Prkar1b gene of the WTC-furue genome. Prkar1b-deficient rats created by the CRISPR/Cas9 system exhibited body tremor. Hippocampal slices from mutant rats showed deficient LTP in the Schaffer collateral–CA1 synapse. Mutant rats also exhibited decreased freezing time following contextual and cued fear conditioning, as well as increased exploratory behavior in the open field. These findings indicate the roles of the RIβ subunit in tremor pathogenesis and contextual and cued fear memory, and suggest that the hippocampal and amygdala roles of this subunit differ between mice and rats and that rats are therefore beneficial for exploring RIβ function. |
| 出版者 |
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出版者 |
Springer Nature |
| 関係URI |
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識別子タイプ |
URI |
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関連識別子 |
https://www.nature.com/articles/s41598-021-81515-x |
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|
関連名称 |
Deficiency of the RIβ subunit of protein kinase A causes body tremor and impaired fear conditioning memory in rats |
| 権利 |
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|
権利情報 |
© The Author(s) 2021 |
| 権利URI |
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|
権利情報 |
http://creativecommons.org/licenses/by/4.0/ |
| DOI |
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|
関連タイプ |
isIdenticalTo |
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|
識別子タイプ |
DOI |
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関連識別子 |
info:doi/10.1038/s41598-021-81515-x |
| 著者版フラグ |
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出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
sr-v11p2039 (1.3 MB)
