| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2021-12-10 |
| タイトル |
|
|
タイトル |
Vitrification of canine ovarian tissues with polyvinylpyrrolidone preserves the survival and developmental capacity of primordial follicles |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
FUJIHARA, Mayako
KANEKO, Takehito
INOUE-MURAYAMA, Miho
|
| 著者(機関) |
|
|
値 |
Wildlife Research Center, Kyoto University |
| 著者(機関) |
|
|
値 |
Division of Science and Engineering, Graduate School of Arts and Science, Iwate University, Department of Chemistry and Biological Sciences, Faculty of Science and Engineering, Iwate University |
| 著者(機関) |
|
|
値 |
Wildlife Research Center, Kyoto University, Wildlife Genome Collaborative Research Group, National Institute for Environmental Studies |
| 登録日 |
|
|
日付 |
2021-12-10 |
| 書誌情報 |
Scientific Reports
巻 9,
p. 3970,
発行日 2019-03-08
|
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
20452322 |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Ovarian tissue cryopreservation combined with immature follicle development can preserve female fertility in wildlife, regardless of age or reproductive timing. To investigate the effects of different cryopreservation methods and cryoprotectants on follicular survival and developmental capacity, ovarian cortical pieces from 15 dogs were cryopreserved by slow freezing or vitrification with different additional cryoprotectants as follows: dimethyl sulfoxide (DMSO), polyvinylpyrrolidone (PVP), combined DMSO and PVP (each at half the concentration of when used independently), or none (control). Cryopreserved ovarian tissues were evaluated by neutral red staining, histology, and xenotransplantation assays. Among cryopreservation conditions tested, vitrification with combined DMSO and PVP significantly improved the maintenance of follicular morphology compared to that in control. Furthermore, ovarian tissues vitrified using this condition maintained follicle morphology and developmental capacity 9 weeks after grafting, as shown by an increased percentage of primary and secondary follicles and a significant decrease in the transition stage from primordial to primary stage follicles 5 and 9 weeks after grafting. In contrast, slow freezing and control groups lost intact follicles by 5 weeks after grafting. The described cryopreservation techniques, which preserve canine follicle development, will build the foundation of ovarian tissue cryopreservation to preserve female fertility in wild canids. |
| 出版者 |
|
|
出版者 |
Springer Nature |
| 関係URI |
|
|
|
識別子タイプ |
URI |
|
|
関連識別子 |
https://www.nature.com/articles/s41598-019-40711-6 |
|
|
関連名称 |
Vitrification of canine ovarian tissues with polyvinylpyrrolidone preserves the survival and developmental capacity of primordial follicles |
| 権利 |
|
|
権利情報 |
© The Author(s) 2019 |
| 権利URI |
|
|
権利情報 |
http://creativecommons.org/licenses/by/4.0/ |
| DOI |
|
|
関連タイプ |
isIdenticalTo |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
info:doi/10.1038/s41598-019-40711-6 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
sr-v9p3970 (1.8 MB)
