| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2021-12-13 |
| タイトル |
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タイトル |
Expression of human mutant cyclin dependent kinase 4, Cyclin D and telomerase extends the life span but does not immortalize fibroblasts derived from loggerhead sea turtle (Caretta caretta) |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
FUKUDA, Tomokazu
EITSUKA, Takahiro
DONAI, Kenichiro
KURITA, Masanori
SAITO, Tomomi
OKAMOTO, Hitoshi
KINOSHITA, Kodzue
KATAYAMA, Masafumi
NITTO, Hiroshi
UCHIDA, Takafumi
ONUMA, Manabu
SONE, Hideko
INOUE-MURAYAMA, Miho
KIYONO, Tohru
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| 著者(機関) |
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値 |
Graduate School of Science and Engineering, Iwate University, Soft-Path Engineering Research Center (SPERC), Iwate University, Wildlife Genome Collaborative Research Group, National Institute for Environmental Studies |
| 著者(機関) |
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値 |
Graduate School of Agricultural Science, Tohoku University |
| 著者(機関) |
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値 |
Graduate School of Agricultural Science, Tohoku University |
| 著者(機関) |
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値 |
Port of Nagoya Public Aquarium |
| 著者(機関) |
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値 |
Usa Marine Biological Institute, Kochi University |
| 著者(機関) |
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値 |
Port of Nagoya Public Aquarium |
| 著者(機関) |
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値 |
Wildlife Research Center, Kyoto University |
| 著者(機関) |
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値 |
Wildlife Genome Collaborative Research Group, National Institute for Environmental Studies, Ecological Genetics Analysis Section, Center for Environmental Biology and Ecosystem, National Institute for Environmental Studies |
| 著者(機関) |
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値 |
Port of Nagoya Public Aquarium |
| 著者(機関) |
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値 |
Graduate School of Agricultural Science, Tohoku University |
| 著者(機関) |
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値 |
Wildlife Genome Collaborative Research Group, National Institute for Environmental Studies, Ecological Genetics Analysis Section, Center for Environmental Biology and Ecosystem, National Institute for Environmental Studies |
| 著者(機関) |
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値 |
Environmental Exposure Research Section, Center for Environmental Risk Research, National Institute for Environmental Studies |
| 著者(機関) |
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値 |
Wildlife Genome Collaborative Research Group, National Institute for Environmental Studies, Wildlife Research Center, Kyoto University |
| 著者(機関) |
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値 |
Division of Carcinogenesis and Cancer Prevention, National Cancer Center Research Institute |
| 登録日 |
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日付 |
2021-12-13 |
| 書誌情報 |
Scientific Reports
巻 8,
p. 9229,
発行日 2018-06-20
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
20452322 |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Conservation of the genetic resources of endangered animals is crucial for future generations. The loggerhead sea turtle (Caretta caretta) is a critically endangered species, because of human hunting, hybridisation with other sea turtle species, and infectious diseases. In the present study, we established primary fibroblast cell lines from the loggerhead sea turtle, and showed its species specific chromosome number is 2n = 56, which is identical to that of the hawksbill and olive ridley sea turtles. We first showed that intensive hybridization among multiple sea turtle species caused due to the identical chromosome number, which allows existence of stable hybridization among the multiple sea turtle species. Expressions of human-derived mutant Cyclin-dependent kinase 4 (CDK4) and Cyclin D dramatically extended the cell culture period, when it was compared with the cell culture period of wild type cells. The recombinant fibroblast cell lines maintained the normal chromosome condition and morphology, indicating that, at the G1/S phase, the machinery to control the cellular proliferation is evolutionally conserved among various vertebrates. To our knowledge, this study is the first to demonstrate the functional conservation to overcome the negative feedback system to limit the turn over of the cell cycle between mammalian and reptiles. Our cell culture method will enable the sharing of cells from critically endangered animals as research materials. |
| 出版者 |
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出版者 |
Springer Nature |
| 関係URI |
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識別子タイプ |
URI |
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関連識別子 |
https://www.nature.com/articles/s41598-018-27271-x |
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関連名称 |
Expression of human mutant cyclin dependent kinase 4, Cyclin D and telomerase extends the life span but does not immortalize fibroblasts derived from loggerhead sea turtle (Caretta caretta) |
| 権利 |
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権利情報 |
© The Author(s) 2018 |
| 権利URI |
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権利情報 |
http://creativecommons.org/licenses/by/4.0/ |
| DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
info:doi/10.1038/s41598-018-27271-x |
| 著者版フラグ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
sr-v8p9229 (8.9 MB)
