| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2021-12-23 |
| タイトル |
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|
タイトル |
Interplay between MPIase, YidC, and PMF during Sec-independent insertion of membrane proteins |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
ENDO, Yuta
SHIMIZU, Yuko
NISHIKAWA, Hanako
SAWASATO, Katsuhiro
NISHIYAMA, Ken-ichi
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| 著者(機関) |
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|
値 |
The United Graduate School of Agricultural Sciences, Iwate University |
| 著者(機関) |
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|
値 |
Department of Biological Chemistry and Food Science, Faculty of Agriculture, Iwate University |
| 著者(機関) |
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|
値 |
Department of Biological Chemistry and Food Science, Faculty of Agriculture, Iwate University |
| 著者(機関) |
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値 |
Department of Biological Chemistry and Food Science, Faculty of Agriculture, Iwate University |
| 著者(機関) |
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|
値 |
The United Graduate School of Agricultural Sciences, Iwate University, Department of Biological Chemistry and Food Science, Faculty of Agriculture, Iwate University |
| 登録日 |
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日付 |
2021-12-23 |
| 書誌情報 |
Life Science Alliance
巻 5,
号 1,
p. e202101162,
発行日 2021-10-12
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
25751077 |
| 抄録 |
|
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内容記述タイプ |
Abstract |
|
内容記述 |
Integral membrane proteins with the N-out topology are inserted into membranes usually in YidC- and PMF-dependent manners. The molecular basis of the various dependencies on insertion factors is not fully understood. A model protein, Pf3-Lep, is inserted independently of both YidC and PMF, whereas the V15D mutant requires both YidC and PMF in vivo. We analyzed the mechanisms that determine the insertion factor dependency in vitro. Glycolipid MPIase was required for insertion of both proteins because MPIase depletion caused a significant defect in insertion. On the other hand, YidC depletion and PMF dissipation had no effects on Pf3-Lep insertion, whereas V15D insertion was reduced. We reconstituted (proteo)liposomes containing MPIase, YidC, and/or F0F1-ATPase. MPIase was essential for insertion of both proteins. YidC and PMF stimulated Pf3-Lep insertion as the synthesis level increased. V15D insertion was stimulated by both YidC and PMF irrespective of the synthesis level. These results indicate that charges in the N-terminal region and the synthesis level are the determinants of YidC and PMF dependencies with the interplay between MPIase, YidC, and PMF. |
| 出版者 |
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出版者 |
Life Science Alliance |
| 関係URI |
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識別子タイプ |
URI |
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関連識別子 |
https://www.life-science-alliance.org/content/5/1/e202101162 |
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関連名称 |
Interplay between MPIase, YidC, and PMF during Sec-independent insertion of membrane proteins |
| 権利 |
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権利情報 |
© 2021 Endo et al. |
| 権利URI |
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権利情報 |
https://creativecommons.org/ licenses/by/4.0/ |
| DOI |
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|
関連タイプ |
isIdenticalTo |
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|
識別子タイプ |
DOI |
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関連識別子 |
info:doi/10.26508/lsa.202101162 |
| 著者版フラグ |
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出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
lsa-v5n1pe202101162 (2.1 MB)
