| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2022-08-19 |
| タイトル |
|
|
タイトル |
Enzyme-linked immunosorbent assay (ELISA) using recombinant Fasciola cathepsin L1 for the diagnosis of human fasciolosis caused by Fasciola hepatica/gigantica hybrid type |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Hybrid type Fasciola |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Human fasciolosis |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Cathepsin L1 |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Enzyme-linked immunosorbent assay |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Sensitivity |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Specificity |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
SUGIYAMA, Takutoshi
ICHIKAWA-SEKI, Madoka
SATO, Hironobu
KOUNOSU, Asuka
TANAKA, Mio
MARUYAMA, Haruhiko
|
| 著者(機関) |
|
|
値 |
Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University |
| 著者(機関) |
|
|
値 |
Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University |
| 著者(機関) |
|
|
値 |
Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University |
| 著者(機関) |
|
|
値 |
Department of Infectious Diseases, Division of Parasitology, Faculty of Medicine, University of Miyazaki |
| 著者(機関) |
|
|
値 |
Department of Parasitology, Institute of Tropical Medicine (NEKKEN), Nagasaki University , Program for Nurturing Global Leaders in Tropical and Emerging Communicable Diseases, Graduate School of Biomedical Sciences, Nagasaki University |
| 著者(機関) |
|
|
値 |
Department of Infectious Diseases, Division of Parasitology, Faculty of Medicine, University of Miyazaki , Center for Animal Disease Control, University of Miyazaki |
| 登録日 |
|
|
日付 |
2022-08-19 |
| 書誌情報 |
Parasitology International
巻 82,
p. 102311,
発行日 2021-02-20
|
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
13835769 |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Recombinant Fasciola cathepsin L-1 (rCatL1) was evaluated in enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of human fasciolosis in Japan. Quality characteristics of the test were accessed by receiver operating characteristic (ROC) analysis, with sera from fasciolosis patients (n = 10), patients with no evidence of parasitic infections (n = 29), and patients with other helminth infections (n = 119). Both the sensitivity and specificity of the test achieved 100% with the control samples. To test the performance of the assay in an authentic situation, 311 serum samples, which had been sent to our laboratory for the diagnosis of parasitic infections from January 2018 to February 2019, were re-assessed using the rCatL1 ELISA. In this case, the sensitivity of the rCatL1 ELISA was 100%, giving positive results to all fasciolosis sera (n = 7), and the specificity was 99.0%, in which three of the 304 non-fasciolosis samples were judged positive. Careful re-examination of the laboratory data and medical imaging of these three patients revealed that one of the patients, who had been diagnosed as having larva migrans syndrome, was judged to be infected with Fasciola, in addition to ascarid nematodes. Thus the true specificity of the assay in the authentic reached 99.3% (302/304). As the rCatL1 ELISA exhibited a highly significant positive likelihood ratio (152.0) and negative likelihood ratio (0.0), calculated from the 311 sample data, this rCatL1 ELISA can be used for routine screening and definitive diagnosis test for fasciolosis in reference laboratories. |
| 抄録（URL） |
|
|
表示名 |
Enzyme-linked immunosorbent assay (ELISA) using recombinant Fasciola cathepsin L1 for the diagnosis of human fasciolosis caused by Fasciola hepatica/gigantica hybrid type |
|
URL |
https://www.sciencedirect.com/science/article/pii/S1383576921000301?via%3Dihub |
| 出版者 |
|
|
出版者 |
Elsevier B.V. |
| 権利 |
|
|
権利情報 |
© 2021 The Authors. Published by Elsevier B.V. |
| 権利URI |
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|
権利情報 |
https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| DOI |
|
|
関連タイプ |
isIdenticalTo |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
info:doi/10.1016/j.parint.2021.102311 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
pi-v82p102311 (4.9 MB)
