| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2022-09-12 |
| タイトル |
|
|
タイトル |
Arf GTPase-activating proteins SMAP1 and AGFG2 regulate the size of Weibel-Palade bodies and exocytosis of von Willebrand factor |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Arf |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
ArfGAP |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Secretory granule |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Exocytosis |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
VWF |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
WPB |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 著者 |
WATANABE, Asano
HATAIDA, Hikari
INOUE, Naoya
KAMON, Kosuke
BABA, Keigo
SASAKI, Kuniaki
KIMURA, Rika
SASAKI, Honoka
EURA, Yuka
NI, Wei-Fen
SHIBASAKI, Yuji
WAGURI, Satoshi
KOKAME, Koichi
SHIBA, Yoko
|
| 登録日 |
|
|
日付 |
2022-09-12 |
|
日付タイプ |
Created |
| 書誌情報 |
en : Biology Open
巻 10,
号 9,
p. bio058789,
発行日 2021-09-01
|
| ISSN |
|
|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
2046-6390 |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Arf GTPase-Activating proteins (ArfGAPs) mediate the hydrolysis of GTP bound to ADP-ribosylation factors (Arfs), which are critical to form transport intermediates. ArfGAPs have been thought to be negative regulators of Arfs; however, accumulating evidence indicates that ArfGAPs are important for cargo sorting and promote membrane traffic. Weibel-Palade bodies (WPBs) are cigar-shaped secretory granules in endothelial cells that contain von Willebrand factor (vWF) as their main cargo. WPB biogenesis at the Golgi was reported to be regulated by Arf and their regulators, but the role of ArfGAPs has been unknown. In this study, we performed siRNA screening of ArfGAPs to investigate the role of ArfGAPs in the biogenesis of WPBs. We found two ArfGAPs, SMAP1 and AGFG2, to be involved in WPB size and vWF exocytosis, respectively. SMAP1 depletion resulted in small-sized WPBs, and the lysosomal inhibitor leupeptin recovered the size of WPBs. The results indicate that SMAP1 functions in preventing the degradation of cigar-shaped WPBs. On the other hand, AGFG2 downregulation resulted in the inhibition of vWF secretion upon Phorbol 12-myristate 13-acetate (PMA) or histamine stimulation, suggesting that AGFG2 plays a role in vWF exocytosis. Our study revealed unexpected roles of ArfGAPs in vWF transport. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
The Company of Biologists Ltd |
|
言語 |
en |
| 権利 |
|
|
言語 |
en |
|
権利情報Resource |
https://creativecommons.org/licenses/by/4.0/ |
|
権利情報 |
Creative Commons Attribution 4.0 International |
| DOI |
|
|
関連タイプ |
isIdenticalTo |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1242/bio.058789 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
bo-v10n9pbio058789 (6.9MB)
