| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2022-09-14 |
| タイトル |
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タイトル |
Virus-Mediated Transient Expression Techniques Enable Functional Genomics Studies and Modulations of Betalain Biosynthesis and Plant Height in Quinoa |
| 言語 |
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言語 |
eng |
| キーワード |
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主題Scheme |
Other |
|
主題 |
apple latent spherical virus |
| キーワード |
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主題Scheme |
Other |
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主題 |
betalain |
| キーワード |
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主題Scheme |
Other |
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主題 |
functional genomics |
| キーワード |
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主題Scheme |
Other |
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主題 |
quinoa |
| キーワード |
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主題Scheme |
Other |
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主題 |
virus-induced gene silencing |
| キーワード |
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主題Scheme |
Other |
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主題 |
virusmediated gene overexpression |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
OGATA, Takuya
TOYOSHIMA, Masami
YAMAMIZO-ODA, Chihiro
KOBAYASHI, Yasufumi
FIJII, Kenichiro
TANAKA, Kojiro
TANAKA, Tsutomu
MIZUKOSHI, Hiroharu
YASUI, Yasuo
NAGATOSHI, Yukari
YOSHIKAWA, Nobuyuki
FUJITA, Yasunari
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| 著者(機関) |
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|
値 |
Biological Resources and Post-harvest Division, Japan International Research Center for Agricultural Sciences (JIRCAS) |
| 著者(機関) |
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値 |
Biological Resources and Post-harvest Division, Japan International Research Center for Agricultural Sciences (JIRCAS) |
| 著者(機関) |
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値 |
Biological Resources and Post-harvest Division, Japan International Research Center for Agricultural Sciences (JIRCAS) |
| 著者(機関) |
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値 |
Biological Resources and Post-harvest Division, Japan International Research Center for Agricultural Sciences (JIRCAS) |
| 著者(機関) |
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値 |
Biological Resources and Post-harvest Division, Japan International Research Center for Agricultural Sciences (JIRCAS) |
| 著者(機関) |
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値 |
Technology Development Group, Actree Corporation |
| 著者(機関) |
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値 |
Technology Development Group, Actree Corporation |
| 著者(機関) |
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値 |
Technology Development Group, Actree Corporation |
| 著者(機関) |
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値 |
Graduate School of Agriculture, Kyoto University |
| 著者(機関) |
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値 |
Biological Resources and Post-harvest Division, Japan International Research Center for Agricultural Sciences (JIRCAS) |
| 著者(機関) |
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値 |
Agri-Innovation Center, Iwate University |
| 著者(機関) |
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|
値 |
Biological Resources and Post-harvest Division, Japan International Research Center for Agricultural Sciences (JIRCAS) |
| 登録日 |
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日付 |
2022-09-14 |
| 書誌情報 |
Frontiers in Plant Science
巻 12,
p. 643499,
発行日 2021-03-18
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
1664462X |
| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
Quinoa (Chenopodium quinoa), native to the Andean region of South America, has been recognized as a potentially important crop in terms of global food and nutrition security since it can thrive in harsh environments and has an excellent nutritional profile. Even though challenges of analyzing the complex and heterogeneous allotetraploid genome of quinoa have recently been overcome, with the whole genome-sequencing of quinoa and the creation of genotyped inbred lines, the lack of technology to analyze gene function in planta is a major limiting factor in quinoa research. Here, we demonstrate that two virus-mediated transient expression techniques, virus-induced gene silencing (VIGS) and virus-mediated overexpression (VOX), can be used in quinoa. We show that apple latent spherical virus (ALSV) can induce gene silencing of quinoa phytoene desaturase (CqPDS1) in a broad range of quinoa inbred lines derived from the northern and southern highland and lowland sub-populations. In addition, we show that ALSV can be used as a VOX vector in roots. Our data also indicate that silencing a quinoa 3,4-dihydroxyphenylalanine 4,5-dioxygenase gene (CqDODA1) or a cytochrome P450 enzyme gene (CqCYP76AD1) inhibits betalain production and that knockdown of a reduced-height gene homolog (CqRHT1) causes an overgrowth phenotype in quinoa. Moreover, we show that ALSV can be transmitted to the progeny of quinoa plants. Thus, our findings enable functional genomics in quinoa, ushering in a new era of quinoa research. |
| 抄録（URL） |
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|
表示名 |
Virus-Mediated Transient Expression Techniques Enable Functional Genomics Studies and Modulations of Betalain Biosynthesis and Plant Height in Quinoa |
|
URL |
https://www.frontiersin.org/articles/10.3389/fpls.2021.643499/full |
| 出版者 |
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出版者 |
Frontiers Media |
| 権利 |
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|
権利情報 |
© 2021 Ogata, Toyoshima, Yamamizo-Oda, Kobayashi, Fujii, Tanaka, Tanaka, Mizukoshi, Yasui, Nagatoshi, Yoshikawa and Fujita. |
| 権利URI |
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権利情報 |
https://creativecommons.org/licenses/by/4.0/ |
| DOI |
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関連タイプ |
isIdenticalTo |
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|
識別子タイプ |
DOI |
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関連識別子 |
info:doi/10.3389/fpls.2021.643499 |
| 著者版フラグ |
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出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
fps-v12p643499 (4.6 MB)
