| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2009-11-04 |
| タイトル |
|
|
タイトル |
Canine neutrophil dysfunction caused by downregulation of β2-integrin expression without mutation |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Integrins |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
CD11b/CD18 |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Congenital neutrophil dysfunction |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Canine leukocyte adhesion deficiency |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Kobayashi, Saori
Sato, Reeko
Abe, Yuya
Inanami, Osamu
Yasui, Hironobu
Omoe, Ktsuhiko
Yasuda, Jun
Hankanga, Careen
Oda, Shinichi
Sasaki, Juso
|
| 著者(機関) |
|
|
値 |
Department of Veterinary Medicine, Faculty of Agriculture, Iwate University |
| 著者(機関) |
|
|
値 |
Department of Veterinary Medicine, Faculty of Agriculture, Iwate University |
| 著者(機関) |
|
|
値 |
Department of Veterinary Medicine, Faculty of Agriculture, Iwate University |
| 著者(機関) |
|
|
値 |
Laboratory of Radiation Biology, Department of Environmental Veterinary Medical Sciences, Graduate School of Veterinary Medicine, Hokkaido University |
| 著者(機関) |
|
|
値 |
Laboratory of Radiation Biology, Department of Environmental Veterinary Medical Sciences, Graduate School of Veterinary Medicine, Hokkaido University |
| 著者(機関) |
|
|
値 |
Department of Veterinary Medicine, Faculty of Agriculture, Iwate University |
| 著者(機関) |
|
|
値 |
Department of Veterinary Medicine, Faculty of Agriculture, Iwate University |
| 著者(機関) |
|
|
値 |
Department of Veterinary Medicine, Faculty of Agriculture, Iwate University |
| 著者(機関) |
|
|
値 |
Department of Animal Science, Faculty of Agriculture, Iwate University |
| 著者(機関) |
|
|
値 |
Department of Veterinary Medicine, Faculty of Agriculture, Iwate University |
| 登録日 |
|
|
日付 |
2009-11-04 |
| 書誌情報 |
Veterinary Immunology and Immunopathology
巻 130,
号 3-4,
p. 187-196,
発行日 2009-01-01
|
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0165-2427 |
| Abstract |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Canine leukocyte adhesion deficiency (CLAD) in Irish setters is caused by genetic defects of leukocyte integrin CD18 leading to recurrent bacterial infections. We report clinical features and analysis of neutrophil function from two mixed-breed canine littermates (one female and one male dog) similar to CLAD. The symptoms of pyogenic infection were first recognized at 3 months of age and since then the patients suffered from recurrent bacterial infections. These clinical findings were strongly suggestive of genetic phagocyte dysfunction. Neutrophil function tests revealed a marked reduction of serum-opsonized zymosan-mediated superoxide production in the two littermates. Neutrophils of the male dog revealed impaired integrin-mediated adherence and phagocytic activity, whereas ability of serum opsonization was normal. There was also a profound decrease of surface expression of CD11b/CD18 and β2-integrin transcript level, detected by real-time RT-PCR without missense mutations unlike CLAD. Immunoblot analysis indicated that protein expression of cytochrome b558 component gp91phox, the cytosolic components p47phox and p67phox of NADPH oxidase components increased profoundly in the male. Our study suggests that decreased transcriptional levels of β2-integrin without mutations, lead to downregulation of surface expression, resulting in multiple defects in adhesion-related neutrophil functions and consequently, recurrent bacterial infections from puppyhood. |
| 出版者 |
|
|
出版者 |
Elsevier |
| 権利 |
|
|
権利情報 |
Copyright © 2009 Elsevier B.V. |
| DOI |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1016/j.vetimm.2009.02.008 |
| 著者版フラグ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
vii-v130n3-4p187-196.pdf (363.4 kB)
