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Genetic characterization of a novel Tib-derived variant of soybean Kunitz trypsin inhibitor detected in wild soybean (Glycine soja)
https://iwate-u.repo.nii.ac.jp/records/9142
https://iwate-u.repo.nii.ac.jp/records/9142a837a4f4-4572-4d95-9b4a-1cd0824bc3bd
| 名前 / ファイル | ライセンス | アクション |
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genome-v47n1p9-14.pdf (352.4 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||
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| 公開日 | 2009-11-17 | |||||||||||||
| タイトル | ||||||||||||||
| タイトル | Genetic characterization of a novel Tib-derived variant of soybean Kunitz trypsin inhibitor detected in wild soybean (Glycine soja) | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | soybean Kunitz trypsin inhibitor | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | polymorphism | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | gene sequence | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | soybean | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | wild soybean | |||||||||||||
| 資源タイプ | ||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||
| 資源タイプ | journal article | |||||||||||||
| 著者 |
Wang, Ke-Jing
× Wang, Ke-Jing
× Yamashita, Tetsuro
× Watanabe, Masao
× Takahata, Yoshihito
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| 著者(機関) | ||||||||||||||
| 値 | Faculty of Agriculture, Iwate University, Institute of Crop Germplasm Resources,Chinese Academy of Agricultural Sciences |
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| 著者(機関) | ||||||||||||||
| 値 | Faculty of Agriculture, Iwate University | |||||||||||||
| 著者(機関) | ||||||||||||||
| 値 | Faculty of Agriculture, Iwate University | |||||||||||||
| 著者(機関) | ||||||||||||||
| 値 | Faculty of Agriculture, Iwate University | |||||||||||||
| 登録日 | ||||||||||||||
| 日付 | 2009-11-17 | |||||||||||||
| 書誌情報 |
GENOME 巻 47, 号 1, p. 9-14, 発行日 2003-01-01 |
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| ISSN | ||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||
| 収録物識別子 | 1480-3321 | |||||||||||||
| Abstract | ||||||||||||||
| 内容記述タイプ | Other | |||||||||||||
| 内容記述 | A novel variant of soybean Kunitz trypsin inhibitor (SKTI) was detected in 530 lines of wild soybean (Glycine soja). This variant showed an intermediate electrophoretic mobility between the Tia and Tic types. In isoelectric focusing polyacrylamide gel electrophoresis gels containing urea, this variant had a similar isoelectric point as that of Tia. The genetic analysis of SKTI bands in F2 seeds from crosses of the new variant type with Tia or Tic type showed that this variant type is controlled by a codominant allele at the SKTI locus. We propose the genetic symbol Tif for this novel variant. When the nucleotide sequence of the Tif gene was compared with those of other types of SKTI genes (Tia, Tib, and Tic), the sequence of Tif was identical to that of Tib with the exception of one A→G transitional mutation occurring at position 676 of Tif. This mutation resulted in an amino acid change from Lys to Glu at the 178 residue. These results suggest that this variant is derived from Tib through a point mutation. In addition, we settled an inconsistency in the number of amino acid differences between Tia and Tib (eight or nine). Analysis of nucleotide and amino acid sequences revealed that Tib was different from Tia by nine amino acids. |
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| 出版者 | ||||||||||||||
| 出版者 | National Research Council Canada | |||||||||||||
| 権利 | ||||||||||||||
| 権利情報 | © 2003 NRC Canada | |||||||||||||
| DOI | ||||||||||||||
| 関連タイプ | isIdenticalTo | |||||||||||||
| 識別子タイプ | DOI | |||||||||||||
| 関連識別子 | 10.1139/G03-087 | |||||||||||||
| 著者版フラグ | ||||||||||||||
| 出版タイプ | VoR | |||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||
