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Identification of genes specifically expressed in androgenesis-derived embryo in rapeseed (Brassica napus L.)
https://iwate-u.repo.nii.ac.jp/records/9145
https://iwate-u.repo.nii.ac.jp/records/91450d270697-9a16-40dc-b929-825221293d5b
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
bs-v58n3p251-259.pdf (1.0 MB)
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| アイテムタイプ | 学術雑誌論文 / Journal Article(1) | |||||||||
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| 公開日 | 2009-12-03 | |||||||||
| タイトル | ||||||||||
| タイトル | Identification of genes specifically expressed in androgenesis-derived embryo in rapeseed (Brassica napus L.) | |||||||||
| キーワード | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Brassica napus | |||||||||
| キーワード | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | microspore embryogenesis | |||||||||
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| 主題Scheme | Other | |||||||||
| 主題 | suppression subtractive | |||||||||
| キーワード | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | hybridization | |||||||||
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| 主題Scheme | Other | |||||||||
| 主題 | ESTs | |||||||||
| キーワード | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | quantitative RT-PCR | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 資源タイプ | journal article | |||||||||
| 著者 |
Tsuwamoto, Ryo
× Tsuwamoto, Ryo
× Takahata, Yoshihito
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| 著者(機関) | ||||||||||
| 値 | Faculty of Agriculture, Iwate University | |||||||||
| 登録日 | ||||||||||
| 日付 | 2009-12-03 | |||||||||
| 書誌情報 |
Breeding science 巻 58, 号 3, p. 251-259, 発行日 2008-01-01 |
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| ISSN | ||||||||||
| 収録物識別子タイプ | ISSN | |||||||||
| 収録物識別子 | 1344-7610 | |||||||||
| Abstract | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | Microspore culture is an efficient system to produce haploid and double-haploid plants, and is a useful model system in developmental research. Although many studies have been carried out to understand the mechanism of microspore embryogenesis, the details of molecular events occurring during its developmental process are unclear. During embryogenesis, a marked morphological change occurs just after globular stage embryos. To gain insight into the developmental process of androgenesis in rapeseed (Brassica napus), we isolated the genes expressed differentially during globular stage by suppression subtractive hybridization (SSH). Of 254 ESTs isolated and sequenced, 82 were singletons and the remaining 172 were assembled into 28 groups based on homology to the identical gene, resulting in the identification of 110 non-redundant EST groups, which are potentially up-regulated genes in androgenesis. Survey of the potential function of the genes showed that 96.4% of these genes were homologous to known genes with 42.7% of unclassified proteins and 13.6% of metabolism. To confirm the expression profiles of isolated genes during androgenesis and zygotic embryogenesis, six selected genes were examined by quantitative RT-PCR analysis. All genes were highly expressed in globular and other stages of androgenesis. The expressions of these genes were confirmed in zygotic embryogenesis. These results serve as a starting point for understanding the mechanism of androgenesis and zygotic embryogenesis. | |||||||||
| 出版者 | ||||||||||
| 出版者 | Japanese Society of Breeding | |||||||||
| 権利 | ||||||||||
| 権利情報 | Copyright (c) 2009 by JAPANESE SOCIETY OF BREEDING | |||||||||
| 権利 | ||||||||||
| 権利情報 | 本文データは学協会の許諾に基づきCiNiiから複製したものである | |||||||||
| 著者版フラグ | ||||||||||
| 出版タイプ | VoR | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
| 異版である | ||||||||||
| 関連タイプ | isVersionOf | |||||||||
| 識別子タイプ | URI | |||||||||
| 関連識別子 | http://ci.nii.ac.jp/Detail/detail.do?LOCALID=ART0008800038&lang=ja | |||||||||
