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Expression of pMGN(-4k)LacZ-neo Gene Introduced into Embryonic Stem (ES) Cells in Chimeric Mouse Fetuses
https://iwate-u.repo.nii.ac.jp/records/9157
https://iwate-u.repo.nii.ac.jp/records/9157a1125487-6729-433b-b3bd-706b4a3eeeaa
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
jmor-v18n3p123-126.pdf (3.0 MB)
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| アイテムタイプ | 学術雑誌論文 / Journal Article(1) | |||||||||||
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| 公開日 | 2010-02-02 | |||||||||||
| タイトル | ||||||||||||
| タイトル | Expression of pMGN(-4k)LacZ-neo Gene Introduced into Embryonic Stem (ES) Cells in Chimeric Mouse Fetuses | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Embryonic stem(ES) cells | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Myogenin gene expression | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | LacZ reporter gene | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Chimeric mouse fetus | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Skeletal-myogenesis | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
| 資源タイプ | journal article | |||||||||||
| 著者 |
Osonoi, Makoto
× Osonoi, Makoto
× Ito, Kazuei
× Takahashi, Jutaro
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| 著者(機関) | ||||||||||||
| 値 | Laboratory of Animal Science, Faculty of Agriculture, Iwate University, Present address: Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University |
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| 著者(機関) | ||||||||||||
| 値 | Laboratory of Animal Science, Faculty of Agriculture, Iwate University | |||||||||||
| 著者(機関) | ||||||||||||
| 値 | Laboratory of Animal Science, Faculty of Agriculture, Iwate University | |||||||||||
| 登録日 | ||||||||||||
| 日付 | 2010-02-02 | |||||||||||
| 書誌情報 |
Journal of Mammalian Ova Research 巻 18, 号 3, p. 123-126, 発行日 2001-01-01 |
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| ISSN | ||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||
| 収録物識別子 | 1341-7738 | |||||||||||
| Abstract | ||||||||||||
| 内容記述タイプ | Other | |||||||||||
| 内容記述 | We attempted to produce chimeric mouse fetuses by using an embryonic stem (ES) cell subline, OM 3, which were transfected with pMGN(-4k)LacZ-neo transgene, and to analyze expression of the transgene in chimeric mouse fetuses. In embryonic days 11-13 (E11-13), no fetuses expressing the transgene were observed. In E14, three of 11 normal fetuses recovered showed the transgene expression. In these chimeric mouse fetuses, the regions expressing the transgene were found in a wide range of fetuses, including the temporal region of the head, trunk from neck to tail, and limbs. It was shown by histochemical analysis that these patterns of transgene expression were specific to skeletal muscles. These results indicated that OM 3 cells might be useful for analyses of mouse skeletal-myogenesis and for the development of basic techniques for skeletal muscles in regenerative medicine. | |||||||||||
| 出版者 | ||||||||||||
| 出版者 | Japanese Society of Mammalian Ova Research | |||||||||||
| 権利 | ||||||||||||
| 権利情報 | Copyright (c) 2001 by Japanese Society of Mammalian Ova Research | |||||||||||
| 著者版フラグ | ||||||||||||
| 出版タイプ | VoR | |||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||
