| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2010-02-03 |
| タイトル |
|
|
タイトル |
Genetic Characterization of a Novel Soybean Kunitz Trypsin Inhibitor |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Glycine soja |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
G. max |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
soybean Kunitz trypsin inhibitor |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
multiple allele |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
gene sequence |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Wang, Ke-Jing
Takahata, Yoshihito
Ito, Kikukatsu
Zhao, Yun-Peng
Tsutsumi, Ken-ichi
Kaizuma, Norihiko
|
| 著者(機関) |
|
|
値 |
Institute of Crop Germplasm Resources, Chinese Academy of Agricultural Science |
| 著者(機関) |
|
|
値 |
Faculty of Agriculture, Iwate University |
| 著者(機関) |
|
|
値 |
Faculty of Agriculture, Iwate University |
| 著者(機関) |
|
|
値 |
Faculty of Agriculture, Iwate University |
| 著者(機関) |
|
|
値 |
Faculty of Agriculture, Iwate University |
| 著者(機関) |
|
|
値 |
Faculty of Agriculture, Iwate University |
| 登録日 |
|
|
日付 |
2010-02-03 |
| 書誌情報 |
Breeding science
巻 51,
号 3,
p. 185-190,
発行日 2001-01-01
|
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1344-7610 |
| Abstract |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Genetic and nucleotide sequence studies were performed on a new variant of soybean Kunitz trypsin inhibitor(SKTI)detected in wild soybean(Glycine soja)and showing a slightly slower electrophoretic mobility than the Tia type. The segregation analysis of SKTI bands in F_2 seeds from crosses of the new variant type with Tia or Tib type showed that the variant type is controlled by an allele codominant to Tia and Tib at an SKTI locus. Nucleotide sequence analysis showed that this variant has 217 amino acids composed of 181 amino acid residues of mature SKTI and extra 25 and 11 amino acids at N- and C-terminal regions, respectively. This sequence was identical to that of Tia(=KTi3), except that a G→A transitional mutation occurred at position 500 of Tia, which results in the translational change from Arg to Lys. The results in the translational change from Arg to Lys. The result of isoelectric forcusing-PAGE coincided with this change. In addition, three nucleotides GCT were inserted at the N-terminus, which leads to an Ala addition in the precursor of this SKTI protein. From these results, we propose the genetic symbol Tie for the new variant SKTI. |
| 出版者 |
|
|
出版者 |
日本育種学会 |
| 権利 |
|
|
権利情報 |
日本育種学会 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
bs-v51n3p185-190.pdf (638.8 kB)
