| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2010-02-04 |
| タイトル |
|
|
タイトル |
A novel functional element in the N-terminal region of Arum concinnatum alternative oxidase is indispensable for catalytic activity of the enzyme in HeLa cells |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Alternative oxidase |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Arum lily |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Thermogenesis |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Inactive isoform |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
N-terminus |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Loss of function |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Kakizaki, Yusuke
Seymour, Roger S.
Ito, Kikukatsu
|
| 著者(機関) |
|
|
値 |
Cryobiofrontier Research Center, Faculty of Agriculture, Iwate University |
| 著者(機関) |
|
|
値 |
Ecology and Evolutionary Biology, University of Adelaide |
| 著者(機関) |
|
|
値 |
Cryobiofrontier Research Center, Faculty of Agriculture, Iwate University |
| 登録日 |
|
|
日付 |
2010-02-04 |
| 書誌情報 |
Biochimica et Biophysica Acta (BBA) - Bioenergetics
巻 1797,
号 1,
p. 20-28,
発行日 2010-01-01
|
| ISSN |
|
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0005-2728 |
| Abstract |
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|
内容記述タイプ |
Other |
|
内容記述 |
Alternative oxidase (AOX) is a quinol-oxygen oxidoreductase, which is known to possess a dicarboxylate diiron reaction center held in structurally postulated α-helical bundle. However, little is known about the structural or functional features of its N-terminal region in any organism, with the exception of a regulatory cysteine residue (CysI) in angiosperm plants. Here, we show that transcripts of two AOX1 isozymes (AcoAOX1a and AcoAOX1b) are coexpressed in thermogenic appendices of Arum concinnatum, while their enzymatic activities seem to be distinct. Namely, AcoAOX1a, an abundantly expressed transcript in vivo, shows an apparent cyanide-insensitive and n-propyl gallate-sensitive respiration during ectopic expression of the protein in HeLa cells, whereas AcoAOX1b exhibits a lower transcript expression, and appears to be totally inactive as AOX at the protein level. Our functional analyses further reveal that an E83K substitution in AcoAOX1b, which is located far upstream of CysI in the N-terminal region, is the cause of this loss of function. These results suggest the presence of a naturally occurring inactive AOX homologue in thermogenic plants. Accordingly, our results further imply that the N-terminal region of the AOX protein functionally contributes to the dynamic activities of respiratory control within the mitochondria. |
| 出版者 |
|
|
出版者 |
Elsevier |
| 権利 |
|
|
権利情報 |
Copyright (c) 2009 Elsevier B.V. |
| DOI |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1016/j.bbabio.2009.07.006 |
| 著者版フラグ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
bbab-v1797n1p20-28.pdf (352.3 kB)
