| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2010-10-07 |
| タイトル |
|
|
タイトル |
Assessment of the congruity of genetic relationships and variation revealed by individual- and bulked-samples-based approaches using RAPD and ISSR markers in Japanese turnip (Brassica rapa ssp. rapa) cultivars |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Brassica rapa ssp. rapa |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Random amplified polymorphic DNA (RAPD) |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Inter-simple sequence repeat (ISSR) |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Individual-based approach |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Bulked-samples-based approach |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Wakui, Kenji
Iwata, Hiroyoshi
Takahashi, Yu
Takahata, Yoshihito
Fujigaki, Junzo
|
| 著者(機関) |
|
|
値 |
Department of Bioproduction Technology, Junior College of Tokyo University of Agriculture |
| 著者(機関) |
|
|
値 |
Department of Information Science and Technology, National Agricultural Research Center |
| 著者(機関) |
|
|
値 |
Faculty of Agriculture, Iwate University |
| 著者(機関) |
|
|
値 |
Faculty of Agriculture, Iwate University |
| 著者(機関) |
|
|
値 |
Department of Bioproduction Technology, Junior College of Tokyo University of Agriculture |
| 登録日 |
|
|
日付 |
2010-10-07 |
| 書誌情報 |
Breeding Science
巻 59,
号 4,
p. 447-452,
発行日 2009-01-01
|
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1344-7610 |
| Abstract |
|
|
内容記述タイプ |
Other |
|
内容記述 |
To find out an efficient and accurate way to estimate the genetic relationship among cultivars having within-variety genetic variation by using different types of approaches, we compared among-varieties genetic similarity estimated based on individual samples and also on bulked population samples of six Japanese turnip (Brassica rapa ssp. rapa) cultivars using RAPD and ISSR markers. In both individual- and bulked-samples-based approaches, 37 individuals were sampled from each cultivar, amplified individually and also as bulked DNA, respectively. Genetic similarity matrices among six cultivars were estimated based on four similarity measures estimated with 125 or 124 polymorphic bands scored in the individual- or bulked-samples-based approach, respectively, and the correlations between the similarity matrices were calculated to evaluate the degree of agreement between matrices. Correlations between similarity matrices calculated based on individuals on the one hand and those calculated based on bulked samples on the other were also high and significant in all combinations of the four similarity measures. Use of computational analysis indicated that 6 to 8 individuals per cultivar were enough to accurately estimate similarity with the individual-based approach. |
| 出版者 |
|
|
出版者 |
JAPANESE SOCIETY OF BREEDING |
| 権利 |
|
|
権利情報 |
Copyright (c) 2009 by JAPANESE SOCIETY OF BREEDING |
| DOI |
|
|
関連タイプ |
isIdenticalTo |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1270/jsbbs.59.447 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
bs-v59n4p447-452.pdf (220.2 kB)
