| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2021-12-08 |
| タイトル |
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|
タイトル |
The transcriptome of wild-type and immortalized corneal epithelial cells |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
FURUYA, Kai
WU, Tao
ORIMOTO, Ai
SUGANO, Eriko
TOMITA, Hiroshi
KIYONO, Tohru
KUROSE, Takahiro
TAKAI, Yoshihiro
FUKUDA, Tomokazu
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| 著者(機関) |
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|
値 |
Graduate School of Science and Engineering, Iwate University |
| 著者(機関) |
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値 |
Graduate School of Science and Engineering, Iwate University |
| 著者(機関) |
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|
値 |
Graduate School of Science and Engineering, Iwate University |
| 著者(機関) |
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|
値 |
Graduate School of Science and Engineering, Iwate University |
| 著者(機関) |
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|
値 |
Graduate School of Science and Engineering, Iwate University |
| 著者(機関) |
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|
値 |
Exploratory Oncology Research and Clinical Trial Center, National Cancer Center |
| 著者(機関) |
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|
値 |
Rohto Pharmaceutical Co., Ltd., Basic Research Development Division |
| 著者(機関) |
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|
値 |
Rohto Pharmaceutical Co., Ltd., Basic Research Development Division |
| 著者(機関) |
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|
値 |
Graduate School of Science and Engineering, Iwate University |
| 登録日 |
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|
日付 |
2021-12-08 |
| 書誌情報 |
Scientific Data
巻 8,
p. 126,
発行日 2021-05-07
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
20524463 |
| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
Cellular immortalization enables indefinite expansion of cultured cells. However, the process of cell immortalization sometimes changes the original nature of primary cells. In this study, we performed expression profiling of poly A-tailed RNA from primary and immortalized corneal epithelial cells expressing Simian virus 40 large T antigen (SV40) or the combination of mutant cyclin-dependent kinase 4 (CDK4), cyclin D1, and telomere reverse transcriptase (TERT). Furthermore, we studied the expression profile of SV40 cells cultured in medium with or without serum. The profiling of whole expression pattern revealed that immortalized corneal epithelial cells with SV40 showed a distinct expression pattern from wild-type cells regardless of the presence or absence of serum, while corneal epithelial cells with combinatorial expression showed an expression pattern relatively closer to that of wild-type cells. |
| 出版者 |
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出版者 |
Springer Nature |
| 関係URI |
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識別子タイプ |
URI |
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関連識別子 |
https://www.nature.com/articles/s41597-021-00908-9 |
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関連名称 |
The transcriptome of wild-type and immortalized corneal epithelial cells |
| 権利 |
|
|
権利情報 |
© The Author(s) 2021 |
| 権利URI |
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|
権利情報 |
http://creativecommons.org/licenses/by/4.0/ |
| DOI |
|
|
関連タイプ |
isIdenticalTo |
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
info:doi/10.1038/s41597-021-00908-9 |
| 著者版フラグ |
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|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
sd-v8p126 (5.6 MB)
