| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2021-12-13 |
| タイトル |
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タイトル |
CdsA is involved in biosynthesis of glycolipid MPIase essential for membrane protein integration in vivo |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
SAWASATO, Katsuhiro
SATO, Ryo
NISHIKAWA, Hanako
IIMURA, Naoki
KAMEMOTO, Yuki
FUJIKAWA, Kohki
YAMAGUCHI, Toshiyuki
KURUMA, Yutetsu
TAMURA, Yasushi
ENDO, Toshiya
UEDA, Takuya
SHIMAMOTO, Keiko
NISHIYAMA, Ken-ichi
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| 著者(機関) |
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値 |
The United Graduate School of Agricultural Sciences, Iwate University |
| 著者(機関) |
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値 |
The United Graduate School of Agricultural Sciences, Iwate University |
| 著者(機関) |
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値 |
The United Graduate School of Agricultural Sciences, Iwate University |
| 著者(機関) |
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値 |
Cryobiofrontier Research Center, Faculty of Agriculture, Iwate University |
| 著者(機関) |
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値 |
Cryobiofrontier Research Center, Faculty of Agriculture, Iwate University |
| 著者(機関) |
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値 |
Bioorganic Research Institute, Suntory Foundation for Life Sciences |
| 著者(機関) |
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値 |
Bioorganic Research Institute, Suntory Foundation for Life Sciences |
| 著者(機関) |
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値 |
Earth-Life Science Institute, Tokyo Institute of Technology |
| 著者(機関) |
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値 |
Faculty of Science, Yamagata University, Yamagata |
| 著者(機関) |
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値 |
Faculty of Life Sciences, Kyoto Sangyo University |
| 著者(機関) |
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値 |
Department of Computational Biology and Medical Sciences Graduate School of Frontier Sciences |
| 著者(機関) |
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値 |
Bioorganic Research Institute, Suntory Foundation for Life Sciences |
| 著者(機関) |
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値 |
The United Graduate School of Agricultural Sciences, Iwate University, Cryobiofrontier Research Center, Faculty of Agriculture, Iwate University, Department of Biological Chemistry and Food Science, Faculty of Agriculture, Iwate University |
| 登録日 |
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日付 |
2021-12-13 |
| 書誌情報 |
Scientific Reports
巻 9,
p. 1372,
発行日 2019-02-04
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
20452322 |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
MPIase is a glycolipid that is involved in membrane protein integration. Despite evaluation of its functions in vitro, the lack of information on MPIase biosynthesis hampered verification of its involvement in vivo. In this study, we found that depletion of CdsA, a CDP-diacylglycerol synthase, caused not only a defect in phospholipid biosynthesis but also MPIase depletion with accumulation of the precursors of both membrane protein M13 coat protein and secretory protein OmpA. Yeast Tam41p, a mitochondrial CDP-diacylglycerol synthase, suppressed the defect in phospholipid biosynthesis, but restored neither MPIase biosynthesis, precursor processing, nor cell growth, indicating that MPIase is essential for membrane protein integration and therefore for cell growth. Consistently, we observed a severe defect in protein integration into MPIase-depleted membrane vesicles in vitro. Thus, the function of MPIase as a factor involved in protein integration was proven in vivo as well as in vitro. Moreover, Cds1p, a eukaryotic CdsA homologue, showed a potential for MPIase biosynthesis. From these results, we speculate the presence of a eukaryotic MPIase homologue. |
| 出版者 |
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出版者 |
Springer Nature |
| 関係URI |
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識別子タイプ |
URI |
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関連識別子 |
https://www.nature.com/articles/s41598-018-37809-8 |
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関連名称 |
CdsA is involved in biosynthesis of glycolipid MPIase essential for membrane protein integration in vivo |
| 権利 |
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権利情報 |
© The Author(s) 2019 |
| 権利URI |
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権利情報 |
http://creativecommons.org/licenses/by/4.0/ |
| DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
info:doi/10.1038/s41598-018-37809-8 |
| 著者版フラグ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
sr-v9p1372 (4.9 MB)
