| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2021-12-15 |
| タイトル |
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タイトル |
Visual Responses of Photoreceptor-Degenerated Rats Expressing Two Different Types of Channelrhodopsin Genes |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
SATO, Masatoshi
SUGANO, Eriko
TABATA, Kitako
SANNOHE, Kei
WATANABE, Yoshito
OZAKI, Taku
TAMAI, Makoto
TOMITA, Hiroshi
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| 著者(機関) |
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|
値 |
Laboratory of Visual Neuroscience, Graduate Course in Biological Sciences, Iwate University Division of Science and Engineering |
| 著者(機関) |
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値 |
Laboratory of Visual Neuroscience, Graduate Course in Biological Sciences, Iwate University Division of Science and Engineering, Soft-Path Engineering Research Center (SPERC), Iwate University Division of Science and Engineering |
| 著者(機関) |
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値 |
Laboratory of Visual Neuroscience, Graduate Course in Biological Sciences, Iwate University Division of Science and Engineering |
| 著者(機関) |
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値 |
Laboratory of Visual Neuroscience, Graduate Course in Biological Sciences, Iwate University Division of Science and Engineering |
| 著者(機関) |
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|
値 |
Laboratory of Visual Neuroscience, Graduate Course in Biological Sciences, Iwate University Division of Science and Engineering |
| 著者(機関) |
|
|
値 |
Laboratory of Visual Neuroscience, Graduate Course in Biological Sciences, Iwate University Division of Science and Engineering, Soft-Path Engineering Research Center (SPERC), Iwate University Division of Science and Engineering |
| 著者(機関) |
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|
値 |
Tohoku University Graduate School of Medicine |
| 著者(機関) |
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|
値 |
Laboratory of Visual Neuroscience, Graduate Course in Biological Sciences, Iwate University Division of Science and Engineering, Soft-Path Engineering Research Center (SPERC), Iwate University Division of Science and Engineering, Clinical Research, Innovation and Education Center, Tohoku University Hospital |
| 登録日 |
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日付 |
2021-12-15 |
| 書誌情報 |
Scientific Reports
巻 7,
p. 41210,
発行日 2017-01-23
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
20452322 |
| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
Optogenetic technologies are expected to be applicable for clinical use in restoring vision. However, the degree of recovered visual function is highly dependent on the function of the chosen optogenetic gene. To investigate the effect on visual function of dual expression of genes with different wavelength sensitivities, we transduced a modified Volvox-derived channelrhodopsin gene (mVChR1) via an adeno-associated virus vector into transgenic rats harbouring the ChR2 gene in retinal ganglion cells. These transgenic rats were given an intraperitoneal injection of N-methyl-N-nitrosourea to induce the degeneration of native photoreceptor cells prior to transduction of mVChR1. Optical coherence tomography images indicated the degeneration of the native photoreceptor cells after the N-methyl-N-nitrosourea injection. Complete loss of function of the native photoreceptor cells was confirmed using electroretinograms. In the ChR2 transgenic rats, visually evoked potentials were clearly detectable in spite of native photoreceptor function abolishment; however the responses were limited to within blue wavelengths. In contrast, the limited wavelength sensitivities were improved by the additional transduction of mVChR1, which exhibited sensitivities to green and red. Thus, the transductions of dual genes encoding channelrhodopsins that exhibit different wavelength sensitivities represents a promising candidate method to expand and to enhance rescued wavelength sensitivities in blind subjects. |
| 出版者 |
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出版者 |
Springer Nature |
| 関係URI |
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識別子タイプ |
URI |
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関連識別子 |
https://www.nature.com/articles/srep41210 |
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関連名称 |
Visual Responses of Photoreceptor- Degenerated Rats Expressing Two Different Types of Channelrhodopsin Genes |
| 権利 |
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権利情報 |
© The Author(s) 2017 |
| 権利URI |
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権利情報 |
http://creativecommons.org/licenses/by/4.0/ |
| DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
info:doi/10.1038/srep41210 |
| 著者版フラグ |
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出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
sr-v7p41210 (2.5 MB)
