| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2022-01-05 |
| タイトル |
|
|
タイトル |
Root sampling and RNA extraction methods for field-based gene expression analysis of soybeans |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Aquaporin |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
RNA extraction |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
root |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
soybean |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
OKAMURA, Shin
Mumtahina, Nabila
SHIMONO, Hiroyuki
MATSUNAMI, Maya
|
| 著者(機関) |
|
|
値 |
Department of Plant Bio Sciences, Faculty of Agriculture, Iwate University |
| 著者(機関) |
|
|
値 |
Graduate School of Arts and Sciences, Division of Agriculture, Iwate University |
| 著者(機関) |
|
|
値 |
Department of Plant Bio Sciences, Faculty of Agriculture, Iwate University, Agri-Innovation Center, Iwate University |
| 著者(機関) |
|
|
値 |
Department of Plant Bio Sciences, Faculty of Agriculture, Iwate University |
| 登録日 |
|
|
日付 |
2022-01-05 |
| 書誌情報 |
Plant Production Science
巻 24,
号 3,
p. 339-345,
発行日 2020-10-21
|
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1343943X |
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
13491008 |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Isolation of an adequate quantity of high-quality RNA is a crucial step in gene expression analysis. Difficulties in sampling and RNA extraction of field-grown crops, especially from roots, are substantial obstacles to understanding actual gene expression in the field. We examined the effectiveness of the RNA extraction method (FRP) using pot- and field-grown soybean roots. High quantity and quality of total RNA could extract by the FRP method from both pot- and field-grown soybean roots at different growth stages. To determine the influence of root washing during the sampling process, roots were washed with water for 5 s, 1 min, and 5 min, then analyzed for aquaporin gene expression levels. Root washing for 5 min affected gene expression of some aquaporins. To obtain RNA that reflects the correct gene expression profile, it is necessary to minimize the effects of the sampling process. |
| 出版者 |
|
|
出版者 |
Taylor & Francis Group |
| 関係URI |
|
|
|
識別子タイプ |
URI |
|
|
関連識別子 |
https://www.tandfonline.com/doi/full/10.1080/1343943X.2020.1834867 |
|
|
関連名称 |
Root sampling and RNA extraction methods for field-based gene expression analysis of soybeans |
| 権利 |
|
|
権利情報 |
© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. |
| 権利URI |
|
|
権利情報 |
http://creativecommons.org/licenses/by/4.0/ |
| DOI |
|
|
関連タイプ |
isIdenticalTo |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
info:doi/10.1080/1343943X.2020.1834867 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
pps-v24n3p339-345 (3.0 MB)
