| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2022-01-11 |
| タイトル |
|
|
タイトル |
Glycated Tryptophan PHP-THβC Incorporated into Various Chicken Embryonic Cells Constitutes Cellular Proteins |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
chicken embryo |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
glycation |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
PHP-THβC |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
protein synthesis |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
tryptophan |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
MAKINO, Ryosuke
ABE, Kayoko
KITA, Kazumi
|
| 著者(機関) |
|
|
値 |
Faculty of Agriculture, Iwate University, Faculty of Agriculture, Ehime University |
| 著者(機関) |
|
|
値 |
Faculty of Agriculture, Iwate University, Graduate School of Systems Life Sciences, Kyushu University |
| 著者(機関) |
|
|
値 |
Faculty of Agriculture, Iwate University |
| 登録日 |
|
|
日付 |
2022-01-11 |
| 書誌情報 |
The journal of poultry science
巻 58,
号 4,
p. 258-262,
発行日 2021
|
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
13490486 |
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
13467395 |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Glycation is a non-enzymatic reaction, and amino acids are glycated by glucose in vivo. Tryptophan is glycated with glucose to form two types of glycated compounds, tryptophan-Amadori product and (1R, 3S)-1-(D-gluco-1, 2, 3, 4, 5-pentahydroxypentyl)-1, 2, 3, 4-tetrahydro-β-carboline-3-carboxylic acid (PHP-THβC). Although PHP-THβC can be incorporated into various chicken embryonic cells, the mechanism of its incorporation into intracellular fluids has not been clarified. In this study, we examined whether PHP-THβC once incorporated into various chicken embryonic cells can combine with proteins. Embryonic cells from the breast muscle, liver, spleen, kidney, proventriculus, gizzard, and skin were prepared and 3H-PHP-THβC was added to the culture medium at final concentrations of 0, 200, 400, 600, and 800 µM to examine the incorporation of PHP-THβC. After 18 h of incubation, radioactivity was measured in the whole-cell and protein fractions of the chicken embryonic cells. As PHP-THβC concentration increased from 0 to 600 µM, its accumulation in the whole-cell fractions of all types of chicken embryonic cells linearly increased and reached the maximum level. The saturated PHP-THβC accumulation in the whole-cell fractions suggests that PHP-THβC could be incorporated into intracellular fluids across cellular membranes by some transporter proteins. As PHP-THβC concentration increased from 0 to 800 µM, its accumulation in the protein fractions of all types of chicken embryonic cells increased in a linear manner and reached a maximum level in the 800 µM PHPTHβC treatment group. This is the first study to indicate that a part of PHP-THβC incorporated into the whole-cell fraction was detected in the protein fraction of various chicken embryonic cells. |
| 抄録（URL） |
|
|
表示名 |
Glycated Tryptophan PHP-THβC Incorporated into Various Chicken Embryonic Cells Constitutes Cellular Proteins |
|
URL |
https://www.jstage.jst.go.jp/article/jpsa/58/4/58_0200076/_article |
| 権利 |
|
|
権利情報 |
Ⓒ 2021 Japan Poultry Science Association |
| 権利URI |
|
|
権利情報 |
https://creativecommons.org/licenses/by-nc-sa/4.0/ |
| NCID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12018767 |
| NCID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11564513 |
| DOI |
|
|
関連タイプ |
isIdenticalTo |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
info:doi/10.2141/jpsa.0200076 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
jps-v58n4p258-262 (310.6 kB)
