| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2022-08-17 |
| タイトル |
|
|
タイトル |
Development of conventional multiplex pcr method for discrimination between dispharynx nasuta and cheilospirura hamulosa ( Nematoda : Acuariidae ) parasitizing poultry |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Cheilospirura hamulosa |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Dispharynx nasuta |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
multiplex PCR |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
nuclear ribosomal 18S-ITS1-5.8S-ITS2-28S DNA |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
poultry |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
BISWAS, Peru Gopal
OHARI, Yuma
MOHANTA, Uday Kumar
ITAGAKI, Tadashi
|
| 著者(機関) |
|
|
値 |
Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University , Department of Pathogenetic Veterinary Science, The United Graduate School of Veterinary Sciences, Gifu University |
| 著者(機関) |
|
|
値 |
Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University , Department of Pathogenetic Veterinary Science, The United Graduate School of Veterinary Sciences, Gifu University |
| 著者(機関) |
|
|
値 |
Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University , Department of Pathogenetic Veterinary Science, The United Graduate School of Veterinary Sciences, Gifu University |
| 著者(機関) |
|
|
値 |
Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University , Department of Pathogenetic Veterinary Science, The United Graduate School of Veterinary Sciences, Gifu University |
| 登録日 |
|
|
日付 |
2022-08-17 |
| 書誌情報 |
Journal of Veterinary Medical Science
巻 83,
号 2,
p. 226-229,
発行日 2021
|
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
13477439 |
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
09167250 |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
The poultry infections caused by Dispharynx nasuta and Cheilospirura hamulosa nematodes are difficult to be diagnosed by fecal examination because of their egg similarity. In this study, we analyzed DNA sequences of nuclear ribosomal 18S-ITS1-5.8S-ITS2-28S region of D. nasuta and C. hamulosa and developed conventional multiplex PCR method using species-specific primers for discriminating between the two species. The method amplified 455-bp and 319-bp fragments specific to D. nasuta and C. hamulosa, respectively, and did not produce them against the other chicken nematode species, Ascaridia galli, Oxyspirura mansoni, Heterakis gallinarum, Heterakis beramporia, and Heterakis indica, suggesting that the multiplex PCR is sensitive and available for species diagnosis. |
| 抄録（URL） |
|
|
表示名 |
Development of conventional multiplex PCR method for discrimination between Dispharynx nasuta and Cheilospirura hamulosa (Nematoda: Acuariidae) parasitizing poultry |
|
URL |
https://www.jstage.jst.go.jp/article/jvms/83/2/83_20-0664/_article/ |
| 出版者 |
|
|
出版者 |
Japanese Society of Veterinary Science |
| 権利 |
|
|
権利情報 |
©2021 The Japanese Society of Veterinary Science |
| 権利URI |
|
|
権利情報 |
https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| NCID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA10796138 |
| NCID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11510037 |
| DOI |
|
|
関連タイプ |
isIdenticalTo |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
info:doi/10.1292/jvms.20-0664 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
jvms-v83n2p226-229 (1.8 MB)
