| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2022-09-20 |
| タイトル |
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|
タイトル |
Development of an optogenetic gene sensitive to daylight and its implications in vision restoration |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
WATANABE, Yoshito
SUGANO, Eriko
TABATA, Kitako
HATAKEYAMA, Akito
SAKAJIRI, Tetsuya
FUKUDA, Tomokazu
OZAKI, Taku
SUZUKI, Tomoya
SAYAMA, Tatsuki
TOMITA, Hiroshi
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| 著者(機関) |
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|
値 |
Graduate Course in Biological Sciences, Iwate University Division of Science and Engineering |
| 著者(機関) |
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|
値 |
Graduate Course in Biological Sciences, Iwate University Division of Science and Engineering |
| 著者(機関) |
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|
値 |
Graduate Course in Biological Sciences, Iwate University Division of Science and Engineering |
| 著者(機関) |
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|
値 |
Graduate Course in Biological Sciences, Iwate University Division of Science and Engineering |
| 著者(機関) |
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|
値 |
Department of Nutrition, Kyushu Nutrition Welfare University |
| 著者(機関) |
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|
値 |
Graduate Course in Biological Sciences, Iwate University Division of Science and Engineering |
| 著者(機関) |
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|
値 |
Graduate Course in Biological Sciences, Iwate University Division of Science and Engineering |
| 著者(機関) |
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|
値 |
Graduate Course in Biological Sciences, Iwate University Division of Science and Engineering |
| 著者(機関) |
|
|
値 |
Graduate Course in Biological Sciences, Iwate University Division of Science and Engineering |
| 著者(機関) |
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値 |
Graduate Course in Biological Sciences, Iwate University Division of Science and Engineering , Clinical Research, Innovation and Education Center, Tohoku University Hospital |
| 登録日 |
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日付 |
2022-09-20 |
| 書誌情報 |
npj Regenerative Medicine
巻 6,
p. 64,
発行日 2021-10-14
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
20573995 |
| 抄録 |
|
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内容記述タイプ |
Abstract |
|
内容記述 |
Optogenetic gene-mediated therapy for restoring vision is thought to be a useful treatment for blind patients. However, light sensitivity achieved using this gene therapy is inferior to that of daylight vision. To increase light sensitivity, we designed three mutants using a bioinformatics approach. Nucleotide sequences encoding two sites in the extracellular loops (ex1, ex3) of mVChR1 close to simulated ion-conducting pathways were replaced by homologous amino acid-encoding sequences of ChR1 or ChR2. The light sensitivity of ex3mV1 was higher than that of mVChR1 at 405–617 nm. Visual responses were restored in Royal College of Surgeons rats with genetically degenerating photoreceptor cells transfected with ex3mV1Co, wherein transmembrane of sixth (TM6) in ex3mV1 was additionally replaced with the corresponding domain of CoChR; these rats responded to light in the order of μW/mm2. Thus, ex3mV1Co might be useful for the restoration of advanced visual function. |
| 抄録（URL） |
|
|
表示名 |
Development of an optogenetic gene sensitive to daylight and its implications in vision restoration |
|
URL |
https://www.nature.com/articles/s41536-021-00177-5 |
| 出版者 |
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|
出版者 |
Nature Research |
| 権利 |
|
|
権利情報 |
© The Author(s) 2021 |
| 権利URI |
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|
権利情報 |
https://creativecommons.org/licenses/by/4.0/ |
| DOI |
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|
関連タイプ |
isIdenticalTo |
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|
識別子タイプ |
DOI |
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|
関連識別子 |
info:doi/10.1038/s41536-021-00177-5 |
| 著者版フラグ |
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|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
npjrm-v6p64 (3.5 MB)
