| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-08-26 |
| タイトル |
|
|
タイトル |
Magnetic control of membrane damage in early endosomes using internalized magnetic nanoparticles |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
magnetic nanoparticles |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
endosomes |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
membrane damage |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
organelle |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 著者 |
YONEKAWA, Yuta
OIKAWA, Kazuki
BAYARKHUU, Boldbaatar
KOBAYASHI, Kizuna
SAITO, Nana
OIKAWA, Ibuki
YAMADA, Ryohei
CHEN, Yu-han
OYANAGI, Koichi
SHIBASAKI, Yuji
KOBAYASHI, Satoru
SHIBA, Yoko
|
| 登録日 |
|
|
日付 |
2025-08-26 |
|
日付タイプ |
Created |
| 書誌情報 |
en : Cell Structure and Function
巻 50,
号 1,
p. 25-39,
ページ数 15,
発行日 2024-12-27
|
| ISSN |
|
|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
1347-3700 |
| ISSN |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
0386-7196 |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Membrane stiffness is essential for cell migration, tumorigenesis, and development; however, the physical properties of intracellular membrane are poorly characterized. In this study, we internalized 20 nm magnetic nanoparticles (MNPs) into MCF7 human breast cancer cells and applied a magnetic field. We investigated whether magnetic field could induce membrane damage of the early endosomes by analyzing the colocalization of MNPs with galectin 3 (Gal3), a cytosolic protein recruited to the lumen of damaged organelles. We first tried to apply magnetic field by electromagnet, and found a direct-current (DC) magnetic field for five minutes increased the colocalization of the MNPs with Gal3, suggesting that the magnetic field damaged the endosomal membrane. We used a neodymium magnet to apply longer and stronger static magnetic fields. The static magnetic field more than 50 mT for five minutes started to damage endosomes, while 100 mT was the most effective. Longer exposure or higher magnetic field strengths did not induce further membrane damage. We confirmed that a Gal3 positive compartment was also positive for the early endosome marker, EEA1, suggesting that the external magnetic field induced membrane damage in the early endosomes. Our results indicate that a static magnetic field can control the membrane damage in early endosomes using internalized MNPs. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Japan Society for Cell Biology |
|
言語 |
en |
| 権利 |
|
|
言語 |
en |
|
権利情報Resource |
https://creativecommons.org/licenses/by/4.0/ |
|
権利情報 |
Creative Commons Attribution 4.0 International |
| 権利 |
|
|
言語 |
en |
|
権利情報 |
© 2025 The Author(s) |
| NCID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA0060007X |
| DOI |
|
|
関連タイプ |
isIdenticalTo |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1247/csf.24037 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 内容注記 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
This article is reproduced under the CC BY 4.0 license from "Magnetic control of membrane damage in early endosomes using internalized magnetic nanoparticles" (https://doi.org/10.1247/csf.24037) by The Author(s). |
|
言語 |
en |
csf-v50i1p25-39.pdf (14.7 MB)
