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A novel complete reconstitution system for membrane integration of the simplest membrane protein
https://iwate-u.repo.nii.ac.jp/records/9196
https://iwate-u.repo.nii.ac.jp/records/9196125e154f-9a28-4257-a98b-c6c39de530e1
名前 / ファイル | ライセンス | アクション |
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bbrc-v394n3p733-736.pdf (2.5 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2010-10-05 | |||||
タイトル | ||||||
タイトル | A novel complete reconstitution system for membrane integration of the simplest membrane protein | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Diacylglycerol | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Integration-stimulating factor | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Liposomes | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Membrane protein integration | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | MPIase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | PURE system | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Nishiyama, Ken-ichi
× Nishiyama, Ken-ichi× Maeda, Masahide× Abe, Masato× Kanamori, Takashi× Shimamoto, Keiko× Kusumoto, Shoichi× Ueda, Takuya× Tokuda, Hajime |
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著者(機関) | ||||||
Institute of Molecular and Cellular Biosciences, The University of Tokyo, (Present address)Cryobiofrontier Research Center, Faculty of Agriculture, Iwate University |
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著者(機関) | ||||||
Suntory Institute for Bioorganic Research | ||||||
著者(機関) | ||||||
Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo | ||||||
著者(機関) | ||||||
Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo | ||||||
著者(機関) | ||||||
Suntory Institute for Bioorganic Research | ||||||
著者(機関) | ||||||
Suntory Institute for Bioorganic Research | ||||||
著者(機関) | ||||||
Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo | ||||||
著者(機関) | ||||||
Institute of Molecular and Cellular Biosciences, The University of Tokyo | ||||||
登録日 | ||||||
日付 | 2010-10-05 | |||||
書誌情報 |
Biochemical and Biophysical Research Communications 巻 394, 号 3, p. 733-736, 発行日 2010-04-01 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0006-291X | |||||
Abstract | ||||||
内容記述タイプ | Other | |||||
内容記述 | A complete reconstitution system for membrane integration of the simplest protein was developed by means of defined factors. A mutant version of Pf3 coat protein, 3L-Pf3 coat, requires neither signal recognition particle/Sec factors nor a membrane potential for its integration into the cytoplasmic membrane of Escherichia coli. Although 3L-Pf3 coat is spontaneously integrated into liposomes composed of phospholipids, diacylglycerol completely blocks such spontaneous integrations at a physiological level. Under the conditions where spontaneous integration does not occur, 3L-Pf3 coat integration was absolutely dependent on a novel integration-stimulating factor. Combination of the PURE system, an in vitro translation system composed of the purified factors involved in translation in E. coli, with liposomes containing the highly purified integration-stimulating factor revealed multiple cycles of 3L-Pf3 coat integration, achieving the complete reconstitution of membrane integration. Based on the function of the factor, we propose that the factor is named MPIase (Membrane Protein Integrase). | |||||
出版者 | ||||||
出版者 | Elsevier Inc. | |||||
権利 | ||||||
権利情報 | (c) 2010 Elsevier Inc. | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.bbrc.2010.03.061 | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa |