| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2010-10-05 |
| タイトル |
|
|
タイトル |
A novel complete reconstitution system for membrane integration of the simplest membrane protein |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Diacylglycerol |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Integration-stimulating factor |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Liposomes |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Membrane protein integration |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
MPIase |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
PURE system |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Nishiyama, Ken-ichi
Maeda, Masahide
Abe, Masato
Kanamori, Takashi
Shimamoto, Keiko
Kusumoto, Shoichi
Ueda, Takuya
Tokuda, Hajime
|
| 著者(機関) |
|
|
値 |
Institute of Molecular and Cellular Biosciences, The University of Tokyo,
(Present address)Cryobiofrontier Research Center, Faculty of Agriculture, Iwate University |
| 著者(機関) |
|
|
値 |
Suntory Institute for Bioorganic Research |
| 著者(機関) |
|
|
値 |
Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo |
| 著者(機関) |
|
|
値 |
Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo |
| 著者(機関) |
|
|
値 |
Suntory Institute for Bioorganic Research |
| 著者(機関) |
|
|
値 |
Suntory Institute for Bioorganic Research |
| 著者(機関) |
|
|
値 |
Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo |
| 著者(機関) |
|
|
値 |
Institute of Molecular and Cellular Biosciences, The University of Tokyo |
| 登録日 |
|
|
日付 |
2010-10-05 |
| 書誌情報 |
Biochemical and Biophysical Research Communications
巻 394,
号 3,
p. 733-736,
発行日 2010-04-01
|
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0006-291X |
| Abstract |
|
|
内容記述タイプ |
Other |
|
内容記述 |
A complete reconstitution system for membrane integration of the simplest protein was developed by means of defined factors. A mutant version of Pf3 coat protein, 3L-Pf3 coat, requires neither signal recognition particle/Sec factors nor a membrane potential for its integration into the cytoplasmic membrane of Escherichia coli. Although 3L-Pf3 coat is spontaneously integrated into liposomes composed of phospholipids, diacylglycerol completely blocks such spontaneous integrations at a physiological level. Under the conditions where spontaneous integration does not occur, 3L-Pf3 coat integration was absolutely dependent on a novel integration-stimulating factor. Combination of the PURE system, an in vitro translation system composed of the purified factors involved in translation in E. coli, with liposomes containing the highly purified integration-stimulating factor revealed multiple cycles of 3L-Pf3 coat integration, achieving the complete reconstitution of membrane integration. Based on the function of the factor, we propose that the factor is named MPIase (Membrane Protein Integrase). |
| 出版者 |
|
|
出版者 |
Elsevier Inc. |
| 権利 |
|
|
権利情報 |
(c) 2010 Elsevier Inc. |
| DOI |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1016/j.bbrc.2010.03.061 |
| 著者版フラグ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
bbrc-v394n3p733-736.pdf (2.5 MB)
