| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2022-01-07 |
| タイトル |
|
|
タイトル |
KAv-1 is Better Suited to Chick Fibroblast Culture than DMEM or 199 Media |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
cell culture |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
cell growth |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
cellular senescence |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
chick fibroblasts |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
DNA damage |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
growth medium |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
KATAYAMA, Masafumi
ONUMA, Manabu
FUKUDA, Tomokazu
|
| 著者(機関) |
|
|
値 |
Center for Environmental Biology and Ecosystem Studies, National Institute for Environmental Studies, Wildlife Genome Collaborative Research Group, National Institute for Environmental Studies |
| 著者(機関) |
|
|
値 |
Center for Environmental Biology and Ecosystem Studies, National Institute for Environmental Studies, Wildlife Genome Collaborative Research Group, National Institute for Environmental Studies |
| 著者(機関) |
|
|
値 |
Wildlife Genome Collaborative Research Group, National Institute for Environmental Studies, Graduate School of Science and Engineering |
| 登録日 |
|
|
日付 |
2022-01-07 |
| 書誌情報 |
The journal of poultry science
巻 58,
号 4,
p. 270-279,
発行日 2021
|
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
13490486 |
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
13467395 |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Cultured cells are a useful resource for poultry scientists, since these cells allow scientists to evaluate biological responses to conditions such as infectious diseases in vitro while mimicking the whole-body response in birds. However avian cell culture requires an optimized basal medium, and there are currently relatively few options for this basal medium (medium 199 and KAv-1). This means that there is still room for the development of an optimal basal medium for avian cell culture. Here we compare KAv-1 medium, Dulbecco's modified Eagle medium (DMEM) and medium 199 during the culture of chick fibroblasts and determine that KAv-1 remains the optimal medium for these assays. Our results show that DNA damage is reduced in fibroblasts cultured in the KAv-1 medium, when compared to both DMEM and Medium 199 and that these cells also display improved growth dynamics in KAv-1 medium when compared to both DMEM and medium 199. To the best of our knowledge, this is the first study to describe a comparative analysis of culture media for avian cells, which would provide useful information for poultry scientists. |
| 抄録（URL） |
|
|
表示名 |
KAv-1 is Better Suited to Chick Fibroblast Culture than DMEM or 199 Media |
|
URL |
https://www.jstage.jst.go.jp/article/jpsa/58/4/58_0200085/_article |
| 出版者 |
|
|
出版者 |
Japan Poultry Science Association |
| 権利 |
|
|
権利情報 |
Ⓒ 2021 Japan Poultry Science Association |
| 権利URI |
|
|
権利情報 |
https://creativecommons.org/licenses/by-nc-sa/4.0/ |
| NCID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11564513 |
| NCID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12018767 |
| DOI |
|
|
関連タイプ |
isIdenticalTo |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
info:doi/10.2141/jpsa.0200085 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
jps-v58n4p270-279 (3.0 MB)
