| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2022-08-26 |
| タイトル |
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|
タイトル |
Characterization of microRNA expression in B cells derived from Japanese black cattle naturally infected with bovine leukemia virus by deep sequencing |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
OCHIAI, Chihiro
MIYAUCHI, Sonoko
KUDO, Yuta
NARUKE, Yuta
YONEYAMA, Syuji
TOMITA, Keisuke
DONGZE, Leng
CHIBA, Yusuke
HIRATA, To-Ichi
ICHIJO, Toshihiro
NAGAI, Kazuya
KOBAYASHI, Sota
YAMADA, Shinji
HIKONO, Hirokazu
MURAKMI, Kenji
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| 著者(機関) |
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|
値 |
Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Iwate University , Food Safety and Consumer Affairs Bureau, Ministry of Agriculture, Forestry and Fisheries |
| 著者(機関) |
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値 |
Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Iwate University , Department of Agriculture, Forestry and Fisheries, Ehime Prefectural Government |
| 著者(機関) |
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値 |
Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Iwate University , Department of Agriculture, Forestry and Fisheries, Iwate Prefectural Government |
| 著者(機関) |
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値 |
Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Iwate University |
| 著者(機関) |
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値 |
Graduate School of Veterinary Sciences, Iwate University |
| 著者(機関) |
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値 |
Graduate School of Veterinary Sciences, Iwate University |
| 著者(機関) |
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値 |
Graduate School of Veterinary Sciences, Iwate University |
| 著者(機関) |
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値 |
Graduate School of Veterinary Sciences, Iwate University |
| 著者(機関) |
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値 |
Field Science Center, Faculty of Agriculture, Iwate University |
| 著者(機関) |
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値 |
Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Iwate University |
| 著者(機関) |
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値 |
Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Iwate University |
| 著者(機関) |
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値 |
Division of Bacterial and Parasitic Disease, National Institute of Animal Health |
| 著者(機関) |
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|
値 |
Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Iwate University , Graduate School of Veterinary Sciences, Iwate University |
| 著者(機関) |
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値 |
Department of Animal Sciences, Teikyo University of Science |
| 著者(機関) |
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|
値 |
Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Iwate University , Graduate School of Veterinary Sciences, Iwate University |
| 登録日 |
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日付 |
2022-08-26 |
| 書誌情報 |
PLoS ONE
巻 16,
号 9,
p. e0256588,
発行日 2021-09-10
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
19326203 |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis (EBL), a malignant B cell lymphoma. However, the mechanisms of BLV-associated lymphomagenesis remain poorly understood. Here, after deep sequencing, we performed comparative analyses of B cell microRNAs (miRNAs) in cattle infected with BLV and those without BLV. In BLV-infected cattle, BLV-derived miRNAs (blv-miRNAs) accounted for 38% of all miRNAs in B cells. Four of these blv-miRNAs (blv-miR-B1-5p, blv-miR-B2-5p, blv-miR-B4-3p, and blv-miR-B5-5p) had highly significant positive correlations with BLV proviral load (PVL). The read counts of 90 host-derived miRNAs (bta-miRNAs) were significantly down-regulated in BLV-infected cattle compared to those in uninfected cattle. Only bta-miR-375 had a positive correlation with PVL in BLV-infected cattle and was highly expressed in the B cell lymphoma tissue of EBL cattle. There were a few bta-miRNAs that correlated with BLV tax/rex gene expression; however, BLV AS1 expression had a significant negative correlation with many of the down-regulated bta-miRNAs that are important for tumor development and/or tumor suppression. These results suggest that BLV promotes lymphomagenesis via AS1 and blv-miRNAs, rather than tax/rex, by down-regulating the expression of bta-miRNAs that have a tumor-suppressing function, and this downregulation is linked to increased PVL. |
| 抄録（URL） |
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|
表示名 |
Characterization of microRNA expression in B cells derived from Japanese black cattle naturally infected with bovine leukemia virus by deep sequencing |
|
URL |
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0256588 |
| 出版者 |
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出版者 |
Public Library of Science |
| 権利 |
|
|
権利情報 |
© 2021 Ochiai et al. |
| 権利URI |
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|
権利情報 |
https://creativecommons.org/licenses/by/4.0/ |
| DOI |
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|
関連タイプ |
isIdenticalTo |
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|
識別子タイプ |
DOI |
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関連識別子 |
info:doi/10.1371/journal.pone.0256588 |
| 著者版フラグ |
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出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
ploso-v19n9pe0256588 (1.7 MB)
