WEKO3
インデックスリンク
アイテム
Cryopreservation of shoot tips of endangered Hayachine-usuyukiso (Leontopodium hayachinense (Takeda) Hara et Kitam.) using a vitrification protocol
https://iwate-u.repo.nii.ac.jp/records/9150
https://iwate-u.repo.nii.ac.jp/records/9150c053afff-292c-4467-8037-daacc4489082
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
pgrcu-v6n2p164-166.pdf (496.9 kB)
|
|
| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2010-01-13 | |||||||||||||||
| タイトル | ||||||||||||||||
| タイトル | Cryopreservation of shoot tips of endangered Hayachine-usuyukiso (Leontopodium hayachinense (Takeda) Hara et Kitam.) using a vitrification protocol | |||||||||||||||
| キーワード | ||||||||||||||||
| 主題Scheme | Other | |||||||||||||||
| 主題 | cryopreservation | |||||||||||||||
| キーワード | ||||||||||||||||
| 主題Scheme | Other | |||||||||||||||
| 主題 | endangered species | |||||||||||||||
| キーワード | ||||||||||||||||
| 主題Scheme | Other | |||||||||||||||
| 主題 | Leontopodium hayachinense (Takeda) Hara et Kitam | |||||||||||||||
| キーワード | ||||||||||||||||
| 主題Scheme | Other | |||||||||||||||
| 主題 | tissue culture; vitrification | |||||||||||||||
| 資源タイプ | ||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||
| 資源タイプ | journal article | |||||||||||||||
| 著者 |
Tanaka, Daisuke
× Tanaka, Daisuke
× Niino, Takao
× Tsuchiya, Yoshiko
× Shirata, Kazuto
× Uemura, Matsuo
|
|||||||||||||||
| 著者(機関) | ||||||||||||||||
| 値 | Genebank, National Institute of Agrobiological Sciences | |||||||||||||||
| 著者(機関) | ||||||||||||||||
| 値 | Genebank, National Institute of Agrobiological Sciences | |||||||||||||||
| 著者(機関) | ||||||||||||||||
| 値 | Graduate School of Agriculture, Iwate University | |||||||||||||||
| 著者(機関) | ||||||||||||||||
| 値 | Genebank, National Institute of Agrobiological Sciences | |||||||||||||||
| 著者(機関) | ||||||||||||||||
| 値 | Graduate School of Agriculture, Iwate University | |||||||||||||||
| 登録日 | ||||||||||||||||
| 日付 | 2010-01-13 | |||||||||||||||
| 書誌情報 |
Plant Genetic Resources: Characterization and Utilization 巻 6, 号 2, p. 164-166, 発行日 2008-01-01 |
|||||||||||||||
| ISSN | ||||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||||
| 収録物識別子 | 1479-2621 | |||||||||||||||
| Abstract | ||||||||||||||||
| 内容記述タイプ | Other | |||||||||||||||
| 内容記述 | Hayachine-usuyukiso (Leontopodium hayachinense) is an alpine plant native to Mt Hayachine. This unique chrysanthemum is listed as an endangered plant by the Department of Conservation, Iwate Prefecture, and as a threatened plant by the Ministry of the Environment, Japan. We successfully cryopreserved the shoot tips from in vitro-grown L. hayachinense shoots using a vitrification protocol. Cold-hardened shoot tips were excised and pre-cultured on a solidified Murashige–Skoog medium containing 0.3M sucrose for 1 d at 58C. The shoot tips were then treated with loading solution for 20 min at 258C, dehydrated in plant vitrification solution 2 for 120 min at 258C and immersed in liquid nitrogen. The survival rate of the vitrified shoot tips was 63.3% after 30 d of regrowth. This protocol appears to be a promising technique for the cryopreservation of in vitro-grown shoots of this endangered plant. |
|||||||||||||||
| 出版者 | ||||||||||||||||
| 出版者 | Cambridge University Press | |||||||||||||||
| 権利 | ||||||||||||||||
| 権利情報 | Copyright © NIAB 2008 | |||||||||||||||
| DOI | ||||||||||||||||
| 関連タイプ | isIdenticalTo | |||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||
| 関連識別子 | 10.1017/S1479262108993175 | |||||||||||||||
| 著者版フラグ | ||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||
