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免疫阻害法を用いた犬血清クレアチンキナーゼBBアイソザイム(CK-BB)測定法の検討
https://iwate-u.repo.nii.ac.jp/records/9332
https://iwate-u.repo.nii.ac.jp/records/933230630618-01e3-4ca3-bed8-6ba7eb8ffa84
名前 / ファイル | ライセンス | アクション |
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jacm-v19n3p79-86.pdf (467.2 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2012-02-14 | |||||
タイトル | ||||||
タイトル | 免疫阻害法を用いた犬血清クレアチンキナーゼBBアイソザイム(CK-BB)測定法の検討 | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | BB isoenzyme | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | canine serum | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | cerebro-neural diseases | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | creatine kinase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | immuno-inhibition method | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | BBアイソザイム | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 犬血清 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 脳神経疾患 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | クレアチンキナーゼ | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 免疫阻害法 | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
矢島, 愛子
× 矢島, 愛子× 小向, 奈津美× 小林, 沙織× 神志那, 弘明× 大石, 明広× 佐藤, れえ子× 安田, 準 |
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著者別名 | ||||||
姓名 | YAJIMA, Aiko | |||||
著者別名 | ||||||
姓名 | KOMUKAI, Natsumi | |||||
著者別名 | ||||||
姓名 | KOBAYASHI, Saori | |||||
著者別名 | ||||||
姓名 | KAMISHINA, Hiroaki | |||||
著者別名 | ||||||
姓名 | OISHI, Akihiro | |||||
著者別名 | ||||||
姓名 | SATO, Reeko | |||||
著者別名 | ||||||
姓名 | YASUDA, Jun | |||||
著者(機関) | ||||||
岩手大学農学部獣医学課程小動物内科学研究室 | ||||||
登録日 | ||||||
日付 | 2012-02-14 | |||||
書誌情報 |
動物臨床医学 巻 19, 号 3, p. 79-86, 発行日 2010-09-20 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1344-6991 | |||||
Abstract | ||||||
内容記述タイプ | Other | |||||
内容記述 | We investigated whether canine serum creatine kinase isoenzyme BB(CK-BB)could be measured by the reagents prepared for human creatine kinase isoenzyme MB (CK-MB), according to an immuno-inhibition method. First,canine serum from a healthy dog and a dog with cerebro-neural-disease was treated respectively with mouse anti-human creatine kinase isoenzyme MM(CK-MM) monoclonal antibody(M antibody) preparation, or with normal mouse immunogloglobulin G(IgG) antibody preparation as a negative control. These treated canine serums and an untreated canine serum were analyzed by agarose electrophoresis/CK enzyme staining.In both dogs, CK-BB and CK-MM bands were detected in the negative controls and in the untreated serum; only the CK-BB band was seen in the M antibody-treated serums.Further, step-wise diluted samples from serum of a healthy dog and a CK-MB control solution were measured by the immuno-inhibition method, which resulted in good linearity of calibration curves. In addition, using the serums of 28 tested dogs including dogs with cerebro-neural disease, the results obtained by the immuno-inhibition method and electrophoresis were compared. There was a statistically significant correlation between the residual CK(which was left uninhibited by M antibodies) determined by the immuno-inhibition method and CK-BB activity determined by electrophoresis. There was also a significant correlation between the values of CK-MM activity determined by both methods. We concluded that measurement of canine serum CK-BB by the immuno-inhibition method developed for human clinics is useful in diagnostic screening for canine cerebro-neural diseases. |
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Abstract | ||||||
内容記述タイプ | Other | |||||
内容記述 | ヒト用免疫阻害法CK一MB測定用試薬を用いて,犬血清CK-BBを測定できるかを検討した。犬血清をマウス抗ヒトCK-MMモノクローナル抗体(M抗体)と正常マウスIgG抗体(陰性コントロール)にそれぞれ感作させた後,無処理犬血清と共にアガロース電気泳動・CK酵素染色した。無処理血清および陰性コントロール血清では,CK-BBとCK-MMが認められた。M抗体処理血清ではCK-BBは出現したがCK-MMは消失して阻害効果を認めた。次に,犬血清とCK-MB活性値が既知のコントロール液の段階希釈検体を免疫阻害法で測定したところ,双方の検量線は良好な直線性が認めら れた。さらに28頭の犬を用いて,免疫阻害法と電気泳動法において,M抗体で阻害されない残存CKとCK-BBの間および両方の測定方法で得たCK-MMの間で良好な相関関係を認めた。犬でも免疫阻害法にてCK-BBを評価できることから,犬の神経疾患のスクリーニング検査に用いることができると考えられた。 |
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出版者 | ||||||
出版者 | 動物臨床医学会 | |||||
その他のタイトル | ||||||
その他のタイトル | Evaluation of Measuring Canine Serum Creatine Kinase Isoenzyme BB by an Immuno-inhibition Method | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |